دورية أكاديمية
Cordycepin suppresses steroidogenic acute regulatory protein expression by reducing SP1 in human granulosa-lutein cells.
| العنوان: | Cordycepin suppresses steroidogenic acute regulatory protein expression by reducing SP1 in human granulosa-lutein cells. |
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| المؤلفون: | Zhang L; Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China., Zhou S; Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China., Bi B; Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China., Wang H; Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China., Fu B; Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China., Guo M; Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China., Luo S; Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China., Cheng JC; Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China., Fang L; Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China. |
| المصدر: | Reproduction (Cambridge, England) [Reproduction] 2024 Sep 26; Vol. 168 (5). Date of Electronic Publication: 2024 Sep 26 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published for the Society for Reproduction and Fertility by BioScientifica Country of Publication: England NLM ID: 100966036 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1741-7899 (Electronic) Linking ISSN: 14701626 NLM ISO Abbreviation: Reproduction Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2004->: Bristol, UK : Published for the Society for Reproduction and Fertility by BioScientifica Original Publication: Cambridge, UK : Journals of Reproduction and Fertility, Ltd. c2001- |
| مواضيع طبية MeSH: | Phosphoproteins*/metabolism , Phosphoproteins*/genetics , Sp1 Transcription Factor*/metabolism , Sp1 Transcription Factor*/genetics , Deoxyadenosines*/pharmacology , Granulosa Cells*/drug effects , Granulosa Cells*/metabolism , Luteal Cells*/drug effects , Luteal Cells*/metabolism, Female ; Humans ; Receptor, Adenosine A1/metabolism ; Receptor, Adenosine A1/genetics ; Cell Line, Tumor ; Signal Transduction/drug effects |
| مستخلص: | In Brief: Cordycepin (COR), a compound derived from Cordyceps, is recognized as an adenosine analog with numerous beneficial effects on human health. However, its impact on steroidogenic acute regulatory protein (STAR) expression in ovarian granulosa cells is not well understood. This study demonstrates that COR downregulates STAR expression by reducing the expression of the SP1 transcription factor. Abstract: Cordycepin (COR), a pure compound of Cordyceps, is known as an adenosine analog that exerts many beneficial effects on human health. The steroidogenesis mediated by ovarian granulosa cells is pivotal in maintaining normal female reproductive function. The steroidogenic acute regulatory protein (STAR) regulates the rate-limiting step in steroidogenesis. COR has been shown to stimulate STAR expression in mouse Leydig cells, the steroidogenic cells in the testes. However, the effect of COR on STAR expression in ovarian granulosa cells remains undetermined. In the present study, we show that treatment with COR downregulates STAR expression in a steroidogenic human granulosa-like tumor cell line, KGN, and primary culture of human granulosa-lutein (hGL) cells obtained from patients undergoing in vitro fertilization. We used specific adenosine receptor (AR) antagonists, and our results reveal that the inhibitory effect of COR on STAR expression is mediated by AR-A1, AR-A2A, and AR-A3. In both KGN and primary hGL cells, COR activates ERK1/2 and AKT signaling pathways, but only activation of ERK1/2 is required for the COR-induced downregulation of STAR expression. In addition, our results demonstrate that COR downregulates STAR expression by reducing the expression of the SP1 transcription factor. These results provide a better understanding of the biological function of COR on STAR expression in the ovary, which may lead to the development of alternative therapeutic approaches for female reproductive disorders. |
| المشرفين على المادة: | 0 (steroidogenic acute regulatory protein) GZ8VF4M2J8 (cordycepin) 0 (Phosphoproteins) 0 (Sp1 Transcription Factor) 0 (Deoxyadenosines) 0 (SP1 protein, human) 0 (Receptor, Adenosine A1) |
| تواريخ الأحداث: | Date Created: 20240812 Date Completed: 20240926 Latest Revision: 20240926 |
| رمز التحديث: | 20240926 |
| DOI: | 10.1530/REP-24-0240 |
| PMID: | 39133156 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1741-7899 |
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| DOI: | 10.1530/REP-24-0240 |