دورية أكاديمية
Effects of Acute High-Altitude Exposure on Morphology and Function of Retinal Ganglion Cell in Mice.
| العنوان: | Effects of Acute High-Altitude Exposure on Morphology and Function of Retinal Ganglion Cell in Mice. |
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| المؤلفون: | Yang Y; Department of Ophthalmology, the Second Hospital of Lanzhou University, Lanzhou, Gansu, People's Republic of China., Han C; Department of Ophthalmology, the Second Hospital of Lanzhou University, Lanzhou, Gansu, People's Republic of China., Sun Y; Department of Ophthalmology, the 940th Hospital of Joint Service Support Forces of the Chinese People's Liberation Army, Lanzhou, Gansu, People's Republic of China., Zhao X; Department of Ophthalmology, the Second Hospital of Lanzhou University, Lanzhou, Gansu, People's Republic of China., Chen Z; Department of Ophthalmology, the Second Hospital of Lanzhou University, Lanzhou, Gansu, People's Republic of China., Zhao L; Cuiying Biomedical Research Center, the Second Hospital of Lanzhou University, Lanzhou, Gansu, People's Republic of China., Li Y; Department of Pathology, Basic Medical School, Ningxia Medical University, Yinchuan, China., Zhang W; Department of Ophthalmology, the Second Hospital of Lanzhou University, Lanzhou, Gansu, People's Republic of China. |
| المصدر: | Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2024 Aug 01; Vol. 65 (10), pp. 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Association For Research In Vision And Ophthalmology (Arvo) Country of Publication: United States NLM ID: 7703701 Publication Model: Print Cited Medium: Internet ISSN: 1552-5783 (Electronic) Linking ISSN: 01460404 NLM ISO Abbreviation: Invest Ophthalmol Vis Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Brookline Ma : Association For Research In Vision And Ophthalmology (Arvo) Original Publication: St. Louis, Mosby. |
| مواضيع طبية MeSH: | Retinal Ganglion Cells*/pathology , Retinal Ganglion Cells*/ultrastructure , Disease Models, Animal* , Electroretinography* , Mice, Inbred C57BL* , Microscopy, Electron, Transmission*, Animals ; Mice ; Male ; Altitude Sickness/physiopathology ; Altitude Sickness/pathology ; Retinal Diseases/physiopathology ; Retinal Diseases/etiology ; Retinal Diseases/pathology ; Altitude ; Acute Disease |
| مستخلص: | Purpose: High altitude retinopathy (HAR) is a retinal functional disorder caused by inadequate adaptation after exposure to high altitude. However, the cellular and molecular mechanisms underlying retinal dysfunction remain elusive. Retinal ganglion cell (RGC) injury is the most important pathological basis for most retinal and optic nerve diseases. Studies focusing on RGC injury after high-altitude exposure (HAE) are scanty. Therefore, the present study sought to explore both functional and morphological alterations of RGCs after HAE. Methods: A mouse model of acute hypobaric hypoxia was established by mimicking the conditions of a high altitude of 5000 m. After HAE for 2, 4, 6, 10, 24, and 72 hours, the functional and morphological alterations of RGCs were assessed using retinal hematoxylin and eosin (H&E) sections, retinal whole mounts, transmission electron microscopy (TEM), and the photopic negative response (PhNR) of the electroretinogram. Results: Compared with the control group, the thickness of the ganglion cell layer and retinal nerve fiber layer increased significantly, RGC loss remained significant, and the amplitudes of a-wave, b-wave, and PhNR were significantly reduced after HAE. In addition, RGCs and their axons exhibited an abnormal ultrastructure after HAE, including nuclear membrane abnormalities, uneven distribution of chromatin in the nucleus, decreased cytoplasmic electron density, widening and vacuolization of the gap between axons, loosening and disorder of myelin sheath structure, widening of the gap between myelin sheath and axon membrane, decreased axoplasmic density, unclear microtubule and nerve fiber structure, and abnormal mitochondrial structure (mostly swollen, with widened membrane gaps and reduced cristae and vacuolization). Conclusions: The study findings confirm that the morphology and function of RGCs are damaged after HAE. These findings lay the foundation for further study of the specific molecular mechanisms of HAR and promote the effective prevention. |
| References: | High Alt Med Biol. 2023 Dec;24(4):296-301. (PMID: 37527184) J Inflamm Res. 2023 Jun 23;16:2645-2660. (PMID: 37383357) Biosci Rep. 2021 Jan 29;41(1):. (PMID: 33393628) Doc Ophthalmol. 2018 Jun;136(3):207-211. (PMID: 29855761) Eye (Lond). 2018 Feb;32(2):370-374. (PMID: 28912514) J Appl Physiol (1985). 2009 Feb;106(2):454-60. (PMID: 19057000) Antioxidants (Basel). 2022 Jan 28;11(2):267. (PMID: 35204150) Front Neurosci. 2019 Mar 15;13:219. (PMID: 30930737) High Alt Med Biol. 2014 Apr;15(1):84-8. (PMID: 24673536) Cells. 2021 Jun 25;10(7):. (PMID: 34201955) Exp Neurobiol. 2018 Feb;27(1):16-27. (PMID: 29535566) Front Physiol. 2022 May 04;13:864222. (PMID: 35600299) High Alt Med Biol. 2024 Jun;25(2):136-139. (PMID: 38436283) Neurochem Int. 2023 Feb;163:105471. (PMID: 36592700) Eur J Ophthalmol. 2012 Jul-Aug;22(4):580-9. (PMID: 22139614) Prog Retin Eye Res. 2023 Mar;93:101169. (PMID: 36736070) J Physiol Sci. 2012 Jul;62(4):309-15. (PMID: 22544448) Prog Retin Eye Res. 2023 Jul;95:101136. (PMID: 36400670) BMC Ophthalmol. 2023 Jan 3;23(1):6. (PMID: 36597056) Arch Ophthalmol. 2004 Mar;122(3):341-6. (PMID: 15006846) High Alt Med Biol. 2008 Summer;9(2):148-57. (PMID: 18578646) Graefes Arch Clin Exp Ophthalmol. 2014 Jan;252(1):43-50. (PMID: 24193351) Vis Neurosci. 1997 Mar-Apr;14(2):339-50. (PMID: 9147485) ISRN Ophthalmol. 2013 Feb 17;2013:182021. (PMID: 24558598) J Ophthalmol. 2012;2012:397178. (PMID: 23133741) Travel Med Infect Dis. 2024 Mar-Apr;58:102689. (PMID: 38295966) Antioxidants (Basel). 2023 Nov 17;12(11):. (PMID: 38001864) Prog Retin Eye Res. 2008 Nov;27(6):622-47. (PMID: 18940262) Clin Ophthalmol. 2008 Dec;2(4):879-89. (PMID: 19668442) Doc Ophthalmol. 2020 Apr;140(2):115-128. (PMID: 31571069) J Travel Med. 2023 Jun 23;30(4):. (PMID: 37166376) Acta Ophthalmol. 2019 May;97(3):279-286. (PMID: 30259682) Exp Eye Res. 2023 Dec;237:109678. (PMID: 37839665) Invest Ophthalmol Vis Sci. 2000 Oct;41(11):3634-42. (PMID: 11006262) Front Neurosci. 2018 Jul 20;12:489. (PMID: 30079010) PLoS One. 2021 Mar 4;16(3):e0246681. (PMID: 33661927) Case Rep Ophthalmol Med. 2021 Aug 10;2021:7076190. (PMID: 34422422) Apoptosis. 2022 Apr;27(3-4):222-232. (PMID: 35088163) Int Ophthalmol. 2020 Dec;40(12):3565-3576. (PMID: 32737731) Doc Ophthalmol. 2005 Jul;111(1):23-31. (PMID: 16502304) |
| تواريخ الأحداث: | Date Created: 20240812 Date Completed: 20240812 Latest Revision: 20240816 |
| رمز التحديث: | 20240816 |
| مُعرف محوري في PubMed: | PMC11323993 |
| DOI: | 10.1167/iovs.65.10.19 |
| PMID: | 39133471 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1552-5783 |
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| DOI: | 10.1167/iovs.65.10.19 |