دورية أكاديمية
THE 72-KDA PROTEIN OF NAEGLERIA FOWLERI PLAYS AN IMPORTANT ROLE IN THE ADHESION OF TROPHOZOITES TO BALB/C MICE NASAL EPITHELIUM.
| العنوان: | THE 72-KDA PROTEIN OF NAEGLERIA FOWLERI PLAYS AN IMPORTANT ROLE IN THE ADHESION OF TROPHOZOITES TO BALB/C MICE NASAL EPITHELIUM. |
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| المؤلفون: | Flores-Suárez B; Laboratorio de Microbiología Ambiental. Proyecto CyMA, UIICSE, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, C.P. 54090, Estado de México, México.; Posgrado en Ciencias Biológicas, Unidad de Posgrado, Edificio A, 1° Piso, Circuito de Posgrados, Ciudad Universitaria, Coyoacán, C.P. 04510, Distrito Federal, México., Bonilla-Lemus P; Laboratorio de Microbiología Ambiental. Proyecto CyMA, UIICSE, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, C.P. 54090, Estado de México, México., Rojas-Hernández S; Laboratorio de Inmunobiología Molecular y Celular, Sección de Estudios de Posgrado e Investigación, Salvador Díaz Mirón esq. Plan de San Luis S/N, Miguel Hidalgo, Casco de Santo Tomas, Ciudad de México 11340, México., Terrazas-Valdés LL; Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, C.P. 54090, Estado de México, México., Carrasco-Yépez MM; Laboratorio de Microbiología Ambiental. Proyecto CyMA, UIICSE, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, C.P. 54090, Estado de México, México. |
| المصدر: | The Journal of parasitology [J Parasitol] 2024 Aug 01; Vol. 110 (4), pp. 360-374. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society of Parasitologists [etc.] Country of Publication: United States NLM ID: 7803124 Publication Model: Print Cited Medium: Internet ISSN: 1937-2345 (Electronic) Linking ISSN: 00223395 NLM ISO Abbreviation: J Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Lawrence, Kans. [etc.] American Society of Parasitologists [etc.] |
| مواضيع طبية MeSH: | Mice, Inbred BALB C* , Naegleria fowleri* , Nasal Mucosa*/parasitology , Protozoan Proteins*/metabolism , Trophozoites* , Central Nervous System Protozoal Infections*/parasitology, Animals ; Mice ; Blotting, Western ; Cell Adhesion ; Female ; Amebiasis/parasitology |
| مستخلص: | Naegleria fowleri is a protozoan that causes primary amebic meningoencephalitis (PAM). The infection occurs when the trophozoites enter the nasal cavity, adhere to the nasal mucosa, invade the epithelium, and migrate until they reach the olfactory bulb. Like other pathogens, there is evidence that the adhesion of N. fowleri to host cells is an important factor in the process of cytopathogenicity and disease progression. However, the factors involved in the adhesion of the pathogen to the cells of the nasal epithelium have not been characterized. The objective of this study was to identify a protein on the surface of N. fowleri, which could act as adhesin to the mouse nasal epithelium in the PAM model. The interaction between proteins of extracts of N. fowleri and cells of the nasal epithelium of BALB/c mice was analyzed using overlay and Western blot assays. A 72-kDa band of N. fowleri interacted directly with epithelial cell proteins, this polypeptide band was purified and analyzed by mass spectrometry. Analysis revealed that polypeptide bands of 72 kDa contained peptides that matched the membrane protein, actin 1 and 2, and Hsp70. Moreover, the N. fowleri extracts resolved in 2D-SDS-PAGE showed that 72-kDa spot interacted with proteins of mouse epithelial cells, which include characteristics of the theoretical data of molecular weight and pH obtained in the analysis by mass spectrometry. Immunofluorescence tests showed that this protein is located on the surface of trophozoites and plays an important role in the adhesion of amoeba either in vitro or in vivo assays, suggesting that this protein contributes during the N. fowleri invasion and migration to the brain, causing primary amoebic meningoencephalitis. (© American Society of Parasitologists 2024.) |
| فهرسة مساهمة: | Keywords: Naegleria fowleri; Epithelial adhesion; Free-living amoebae; Invasion mechanisms; Parasitism; Primary amebic meningoencephalitis; Protein interaction; Protozoan |
| المشرفين على المادة: | 0 (Protozoan Proteins) |
| تواريخ الأحداث: | Date Created: 20240812 Date Completed: 20240812 Latest Revision: 20240812 |
| رمز التحديث: | 20240813 |
| DOI: | 10.1645/22-86 |
| PMID: | 39134068 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1937-2345 |
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| DOI: | 10.1645/22-86 |