دورية أكاديمية
A novel immunofluorescence study of Lingual Salivary Glands in the Egyptian Tortoise (Testudo kleinmanni) and its ecological significance.
| العنوان: | A novel immunofluorescence study of Lingual Salivary Glands in the Egyptian Tortoise (Testudo kleinmanni) and its ecological significance. |
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| المؤلفون: | Rashwan AM; Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt; Laboratory of Life science frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan., El-Gendy SAA; Anatomy and Embryology Department, Faculty of Veterinary Medicine, Alexandria University, Alexandria 21944, Egypt., El-Mansi AA; Biology Department, Faculty of Science, King Khalid University, Abha 61413, Saudi Arabia., Eldesoqui MB; Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, Diriyah, Riyadh 13713, Saudi Arabia; Department of Human Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt., Alsafy MAM; Anatomy and Embryology Department, Faculty of Veterinary Medicine, Alexandria University, Alexandria 21944, Egypt. Electronic address: mohamed.alsafy@alexu.edu.eg. |
| المصدر: | Tissue & cell [Tissue Cell] 2024 Oct; Vol. 90, pp. 102517. Date of Electronic Publication: 2024 Aug 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 0214745 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-3072 (Electronic) Linking ISSN: 00408166 NLM ISO Abbreviation: Tissue Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Edinburgh : Churchill Livingstone Original Publication: Edinburgh, Oliver & Boyd. |
| مواضيع طبية MeSH: | Turtles*/metabolism , Salivary Glands*/metabolism , Salivary Glands*/cytology , Fluorescent Antibody Technique*, Animals ; Tongue/cytology ; Tongue/metabolism ; Egypt |
| مستخلص: | The Egyptian tortoise (Testudo kleinmanni) is remarkably adapted to its harsh desert environment, a characteristic that is crucial for its survival under extreme conditions. This study was aimed at providing a deeper understanding of the lingual salivary gland structures in the Egyptian tortoise and examining how these structures help the tortoise manage hydration and nutrition in arid conditions. Utilizing a combination of light microscopy and immunofluorescence, this research introduced pioneering methods involving seven different antibodies, marking a first in the study of reptilian salivary glands. Our investigations categorized the tortoise's salivary glands into papillary and non-papillary types. The papillary glands were further classified into superficial, deep, interpapillary, and intraepithelial salivary glands, while non-papillary glands included superficial and deep lingual types. Structurally, these glands are organized into lobules, delineated by interlobular septa, and are equipped with a duct system comprising interlobular, intercalated, and main excretory ducts with gland openings on the tongue's surface and the papillae surfaces. Notably, the superficial glands displayed both tubuloalveolar and acinar configurations, whereas the deep lingual glands were exclusively acinar. Immunofluorescence results indicated that α-smooth muscle actin (α-SMA) was prevalent in myoepithelial cells, myofibroblasts, and blood vessels, suggesting their integral role in glandular function and support. E-cadherin was predominantly found in epithelial cells, enhancing cell adhesion and integrity, which are critical for efficient saliva secretion. Importantly, Mucin 1 (MUC1) and Mucin 5B (MUC5B) staining revealed that most glands were mucous in nature, with MUC5B specifically marking mucin within secretory cells, confirming their primary function in mucous secretion. PDGFRα and CD34 highlighted the presence of telocytes and stromal cells within the glandular and interlobular septa, indicating a role in structural organization and possibly in regenerative processes. Cytokeratin 14 expression was noted in the basal cells of the glands, underscoring its role in upholding the structural foundation of the epithelial barrier. In conclusion, this detailed morphological and immunological characterization of the Egyptian tortoise's salivary glands provides new insights into their complex structure and essential functions. These findings not only enhance our understanding of reptilian physiology but also underline the critical nature of salivary glands in supporting life in arid environments. This study's innovative use of a broad range of immunofluorescence markers opens new avenues for further research into the adaptive mechanisms of reptiles. Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interests. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: E-cadherin; MUC1; MUC5B; Salivary gland; Telocytes; Tortoise; α-SMA |
| تواريخ الأحداث: | Date Created: 20240813 Date Completed: 20240902 Latest Revision: 20240902 |
| رمز التحديث: | 20240902 |
| DOI: | 10.1016/j.tice.2024.102517 |
| PMID: | 39137537 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1532-3072 |
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| DOI: | 10.1016/j.tice.2024.102517 |