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Effect of mutation of phaC on carbon supply, extracellular polysaccharide production, and pathogenicity of Xanthomonas oryzae pv. oryzae.

التفاصيل البيبلوغرافية
العنوان: Effect of mutation of phaC on carbon supply, extracellular polysaccharide production, and pathogenicity of Xanthomonas oryzae pv. oryzae.
المؤلفون: Gao X; College of Plant Protection, Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Hainan University), Ministry of Education, Haikou, 570228, China., Tan Z; College of Plant Protection, Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Hainan University), Ministry of Education, Haikou, 570228, China., Fang Y; College of Plant Protection, Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Hainan University), Ministry of Education, Haikou, 570228, China., Xie Q; College of Plant Protection, Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Hainan University), Ministry of Education, Haikou, 570228, China., Liu W; College of Plant Protection, Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Hainan University), Ministry of Education, Haikou, 570228, China., Tao J; College of Tropical Crops, Hainan University, Haikou, 570228, China., Miao W; College of Plant Protection, Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Hainan University), Ministry of Education, Haikou, 570228, China. Miao@hainanu.edu.cn., Jin P; College of Plant Protection, Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Hainan University), Ministry of Education, Haikou, 570228, China. jinpengfei@hainanu.edu.cn.
المصدر: Scientific reports [Sci Rep] 2024 Aug 13; Vol. 14 (1), pp. 18781. Date of Electronic Publication: 2024 Aug 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Xanthomonas*/pathogenicity , Xanthomonas*/genetics , Xanthomonas*/metabolism , Oryza*/microbiology , Carbon*/metabolism , Plant Diseases*/microbiology , Polysaccharides, Bacterial*/metabolism , Polysaccharides, Bacterial*/biosynthesis , Bacterial Proteins*/genetics , Bacterial Proteins*/metabolism, Virulence/genetics ; Mutation ; Gene Expression Regulation, Bacterial ; Polyhydroxyalkanoates/biosynthesis ; Polyhydroxyalkanoates/metabolism ; Nicotiana/microbiology ; Plant Leaves/microbiology
مستخلص: Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight in rice. Polyhydroxyalkanoates (PHAs) consitute a diverse group of biopolyesters synthesized by bacteria under nutrient-limited conditions. The phaC gene is important for PHA polymerization. We investigated the effects of phaC gene mutagensis in Xoo strain PXO99 A . The phaC gene knock-out mutant exhibited reduced swarming ability relative to that of the wild-type. Under conditions where glucose was the sole sugar source, extracellular polysaccharide (EPS) production by ΔphaC declined by 44.8%. ΔphaC showed weak hypersensitive response (HR) induction in the leaves of non-host Nicotiana tabacum, concomitant with downregulation of hpa1 gene expression. When inoculated in rice leaves by the leaf-clipping method, ΔphaC displayed reduced virulence in terms of lesion length compared with the wild-type strain. The complemented strain showed no significant difference from the wild-type strain, suggesting that the deletion of phaC in Xoo induces significant alterations in various physiological and biological processes. These include bacterial swarming ability, EPS production, transcription of hrp genes, and glucose metabolism. These changes are intricately linked to the energy utilization and virulence of Xoo during plant infection. These findings revealed involvement of phaC in Xoo is in the maintaining carbon metabolism by functioning in the PHA metabolic pathway.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 322RC591 Natural Science Foundation of Hainan Province; 31960552 National Natural Science Foundation of China
فهرسة مساهمة: Keywords: Xanthomonas oryzae pv. oryzae; Biological functions; PHA synthase (phaC gene); Polyhydroxyalkanoate; Rice; Virulence
المشرفين على المادة: 7440-44-0 (Carbon)
0 (Polysaccharides, Bacterial)
0 (Bacterial Proteins)
0 (Polyhydroxyalkanoates)
SCR Organism: Xanthomonas oryzae
تواريخ الأحداث: Date Created: 20240813 Date Completed: 20240813 Latest Revision: 20240816
رمز التحديث: 20240816
مُعرف محوري في PubMed: PMC11322296
DOI: 10.1038/s41598-024-69621-y
PMID: 39138326
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-69621-y