دورية أكاديمية
Comprehensive metabolomics and chemometrics unravel potential anti-diabetic metabolites of pumpkin (Cucurbita pepo L.) fruits through UPLC-QqQ-MS and GC-MS analyses.
| العنوان: | Comprehensive metabolomics and chemometrics unravel potential anti-diabetic metabolites of pumpkin (Cucurbita pepo L.) fruits through UPLC-QqQ-MS and GC-MS analyses. |
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| المؤلفون: | Abdelmonsef M; Department of Pharmacognosy, Faculty of Pharmacy, Damanhur University, Egypt., Shawky E; Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Egypt. Electronic address: eman.m.shawky@alexu.edu.eg., Ghareeb DA; Biological screening and preclinical trial laboratory, Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, Egypt., El Naggar EMB; Department of Pharmacognosy, Faculty of Pharmacy, Damanhur University, Egypt., El Newehy NM; Department of Pharmacognosy, Faculty of Pharmacy, Damanhur University, Egypt. |
| المصدر: | Food research international (Ottawa, Ont.) [Food Res Int] 2024 Sep; Vol. 192, pp. 114771. Date of Electronic Publication: 2024 Jul 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science Country of Publication: Canada NLM ID: 9210143 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7145 (Electronic) Linking ISSN: 09639969 NLM ISO Abbreviation: Food Res Int Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Ottawa, Ontario, Canada : Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science, c1992- |
| مواضيع طبية MeSH: | Cucurbita*/chemistry , Fruit*/chemistry , Metabolomics*/methods , Hypoglycemic Agents*/pharmacology , Hypoglycemic Agents*/analysis , Gas Chromatography-Mass Spectrometry* , Plant Extracts*/pharmacology , Plant Extracts*/chemistry, Chromatography, High Pressure Liquid ; Glycoside Hydrolase Inhibitors/pharmacology ; Seeds/chemistry |
| مستخلص: | This comprehensive study explores the phytoconstituents of different parts of pumpkin (Cucurbita pepo) including flesh, peel, seeds, pumpkin juice, and pumpkin seed oil. Utilizing advanced analytical techniques including UPLC-QqQ-MS and GC-TSQ-MS combined with multivariate statistical analysis, 94 distinct chromatographic peaks from various chemical classes were annotated. Predominant classes included phenolic acids, flavonoids, cucurbitacins, amino acids, triterpenoids, fatty acids, sterols, carotenoids, and other compounds. For more comprehensive chemical profiling of the tested samples, fractionation of the different parts of the fruit was attempted through successive solvent extraction. The unsaponifiable part of the oils, analyzed by GC, showed that the phytosterols, namely ß-sitosterol, and stigmasterol are in the majority. All pumpkin extracts showed significant inhibition of carbohydrase enzymes and glucose uptake promotion by cells. Pumpkin flesh butanol fraction exhibited potent α-glucosidase inhibition, while pumpkin defatted seed methylene chloride fraction showed strong α-amylase inhibition. Additionally, pumpkin seed oil and defatted seed petroleum ether fraction demonstrated high glucose uptake activity. Bioactive metabolites including vaccenic acid, sinapic acid, kuguacin G, luteolin hexoside, delta-7-avenasterol, cucurbitosides and others were unveiled through OPLS multivariate models elucidating the anti-diabetic potential of pumpkin. These findings support the use of pumpkin as a functional food, offering insights into its mechanisms of action in diabetes management. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antidiabetic activity; GC-TSQ-MS; Pumpkin fruit parts; UPLC-QqQ-MS |
| المشرفين على المادة: | 0 (Hypoglycemic Agents) 0 (Plant Extracts) 0 (Glycoside Hydrolase Inhibitors) |
| تواريخ الأحداث: | Date Created: 20240815 Date Completed: 20240815 Latest Revision: 20240815 |
| رمز التحديث: | 20240816 |
| DOI: | 10.1016/j.foodres.2024.114771 |
| PMID: | 39147478 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-7145 |
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| DOI: | 10.1016/j.foodres.2024.114771 |