دورية أكاديمية
Identification and characterization of a novel type II toxin-antitoxin system in Aeromonas veronii.
| العنوان: | Identification and characterization of a novel type II toxin-antitoxin system in Aeromonas veronii. |
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| المؤلفون: | Ji C; State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, P. R. China., He T; State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, P. R. China., Wu B; State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, P. R. China., Cao X; State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, P. R. China., Fan X; State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, P. R. China., Liu X; State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, P. R. China., Li X; State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, P. R. China., Yang M; State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, P. R. China., Wang J; State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, P. R. China., Xu L; State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, P. R. China., Hu S; State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, P. R. China., Xia L; State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, P. R. China., Sun Y; State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, P. R. China. sunyj@hunnu.edu.cn. |
| المصدر: | Archives of microbiology [Arch Microbiol] 2024 Aug 17; Vol. 206 (9), pp. 381. Date of Electronic Publication: 2024 Aug 17. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0410427 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-072X (Electronic) Linking ISSN: 03028933 NLM ISO Abbreviation: Arch Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin, New York, Springer-Verlag. |
| مواضيع طبية MeSH: | Aeromonas veronii*/genetics , Aeromonas veronii*/metabolism , Toxin-Antitoxin Systems*/genetics , Bacterial Proteins*/genetics , Bacterial Proteins*/metabolism, Bacterial Toxins/metabolism ; Bacterial Toxins/genetics ; Operon ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Escherichia coli/drug effects ; Antitoxins/genetics ; Antitoxins/metabolism ; Gene Expression Regulation, Bacterial |
| مستخلص: | The bacterial type II toxin-antitoxin (TA) system is a rich genetic element that participates in various physiological processes. Aeromonas veronii is the main bacterial pathogen threatening the freshwater aquaculture industry. However, the distribution of type II TA system in A. veronii was seldom documented and its roles in the life activities of A. veronii were still unexplored. In this study, a novel type II TA system AvtA-AvtT was predicted in a fish pathogen Aeromonas veronii biovar sobria with multi-drug resistance using TADB 2.0. Through an Escherichia coli host killing and rescue assay, we demonstrated that AvtA and AvtT worked as a genuine TA system, and the predicted toxin AvtT actually functioned as an antitoxin while the predicted antitoxin AvtA actually functioned as a toxin. The binding ability of AvtA with AvtT proteins were confirmed by dot blotting analysis and co-immunoprecipitation assay. Furthermore, we found that the toxin and antitoxin labelled with fluorescent proteins were co-localized. In addition, it was found that the transcription of AvtAT bicistronic operon was repressed by the AvtAT protein complex. Deletion of avtA gene and avtT gene had no obvious effect on the drug susceptibility. This study provides first characterization of type II TA system AvtA-AvtT in aquatic pathogen A. veronii. (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
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| معلومات مُعتمدة: | 23A0053 Scientific Research Fund of Hunan Provincial Education Department; 2020JJ4055 Hunan Provincial Natural Science Foundation; XCN202137 Systematic Survey of Agricultural Microbial Germplasm Resources in Hunan Province |
| فهرسة مساهمة: | Keywords: Aeromonas veronii; Antibiotic resistance; AvtA-AvtT; Type II TA system |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (Bacterial Toxins) 0 (Antitoxins) |
| تواريخ الأحداث: | Date Created: 20240817 Date Completed: 20240817 Latest Revision: 20240817 |
| رمز التحديث: | 20240818 |
| DOI: | 10.1007/s00203-024-04101-5 |
| PMID: | 39153128 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1432-072X |
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| DOI: | 10.1007/s00203-024-04101-5 |