دورية أكاديمية
Novel heterologously expressed protein, AjPSPLP-3, derived from Apostichopus japonicus exhibits cell proliferation and migration activities.
| العنوان: | Novel heterologously expressed protein, AjPSPLP-3, derived from Apostichopus japonicus exhibits cell proliferation and migration activities. |
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| المؤلفون: | Wang W; Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250013, China., Meng Y; Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250013, China., Yin X; Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250013, China., Zhao P; Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250013, China., Wang M; Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250013, China., Ren J; Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250013, China., Zhang J; Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250013, China., Zhang L; Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250013, China; State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China., Cui Y; School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China. Electronic address: cuiyunqian@126.com., Xia X; Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250013, China. Electronic address: xiaxk@sdas.org. |
| المصدر: | Protein expression and purification [Protein Expr Purif] 2024 Dec; Vol. 224, pp. 106577. Date of Electronic Publication: 2024 Aug 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 9101496 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0279 (Electronic) Linking ISSN: 10465928 NLM ISO Abbreviation: Protein Expr Purif Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Orlando, FL : Academic Press Original Publication: San Diego : Academic Press, c1990- |
| مواضيع طبية MeSH: | Stichopus*/genetics , Stichopus*/chemistry , Cell Proliferation*/drug effects , Cell Movement*/drug effects, Animals ; Humans ; Mice ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/chemistry ; Recombinant Fusion Proteins/pharmacology ; Recombinant Fusion Proteins/metabolism ; Recombinant Fusion Proteins/isolation & purification ; Cloning, Molecular ; Amino Acid Sequence ; Cell Line ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Maltose-Binding Proteins/genetics ; Maltose-Binding Proteins/chemistry ; Maltose-Binding Proteins/metabolism ; HaCaT Cells |
| مستخلص: | Developing more effective bioactive ingredients of natural origin is imperative for promoting wound healing. Sea cucumbers have long enjoyed a good reputation as both food delicacies and traditional medicines. In this study, we heterogeneously expressed a Apostichopus japonicus derived novel protein AjPSPLP-3, which exhibits a theoretical molecular weight of 13.034 kDa, through fusion with maltose binding protein (MBP). AjPSPLP-3 contains a strict CXXCXC motif, nine extremely conserved cysteine residues and two highly conserved cysteine residues. The predicted structure of AjPSPLP-3 consists of random coil and nine β-sheets, Cys 30 -Cys 67 , Cys 38 -Cys 58 , Cys 53 -Cys 90 , Cys 56 -Cys 66 , and Cys 81 -Cys 102 participating in the formation of five pairs of disulfide bonds. In vitro experiments conducted on HaCaT cells proved that AjPSPLP-3 and MBP-fused AjPSPLP-3 significantly contribute to HaCaT cells proliferation and migration without exhibiting hemolytic activity on murine erythrocytes. Specifically, treatment with 10 μmol/L MBP-fused AjPSPLP-3 protein increased the viability of HaCaT cells by 12.28 % (p < 0.001), while treatment with 10 μmol/L AjPSPLP-3 protein increased viability of HaCaT cells by 6.01 % (p < 0.01). Furthermore, wound closure of MBP-fused AjPSPLP-3 and AjPSPLP-3 were 22.51 % (p < 0.01) and 7.32 % (p < 0.05) higher than that of the control groups in HaCaT cells following 24 h of incubation. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: AjPSPLP-3; Apostichopus japonicus; Cell migration; Cell proliferation; Heterologous expression |
| المشرفين على المادة: | 0 (Recombinant Fusion Proteins) 0 (Maltose-Binding Proteins) |
| تواريخ الأحداث: | Date Created: 20240817 Date Completed: 20240826 Latest Revision: 20240831 |
| رمز التحديث: | 20240901 |
| DOI: | 10.1016/j.pep.2024.106577 |
| PMID: | 39153562 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-0279 |
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| DOI: | 10.1016/j.pep.2024.106577 |