دورية أكاديمية
Production Process Optimization of Recombinant Erwinia carotovora l-Asparaginase II in Escherichia coli Fed-Batch Cultures and Analysis of Antileukemic Potential.
العنوان: | Production Process Optimization of Recombinant Erwinia carotovora l-Asparaginase II in Escherichia coli Fed-Batch Cultures and Analysis of Antileukemic Potential. |
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المؤلفون: | de Andrade BC; National Institute of Science and Technology in Tuberculosis, Research Center for Molecular and Functional Biology, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 90619-900, Brazil.; Graduate Program in Medicine and Health Sciences, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 90619-900, Brazil., Renard G; Quatro G Pesquisa & Desenvolvimento Ltd., Porto Alegre, Rio Grande do Sul 90619-900, Brazil., Gennari A; Food Biotechnology Laboratory, Biotechnology Graduate Program, University of Vale do Taquari (UNIVATES), Lajeado, Rio Grande do Sul 95914-014, Brazil., Artico LL; Centro Infantil Boldrini, Campinas, São Paulo 13083-210, Brazil.; Graduate Program in Genetics and Molecular Biology, Biology Institute, State University of Campinas, Campinas, São Paulo 13083-970, Brazil., Júnior JRT; Centro Infantil Boldrini, Campinas, São Paulo 13083-210, Brazil.; Graduate Program in Genetics and Molecular Biology, Biology Institute, State University of Campinas, Campinas, São Paulo 13083-970, Brazil., Kuhn D; Food Biotechnology Laboratory, Biotechnology Graduate Program, University of Vale do Taquari (UNIVATES), Lajeado, Rio Grande do Sul 95914-014, Brazil., Salles PPZ; Centro Infantil Boldrini, Campinas, São Paulo 13083-210, Brazil.; Graduate Program in Genetics and Molecular Biology, Biology Institute, State University of Campinas, Campinas, São Paulo 13083-970, Brazil., Volken de Souza CF; Food Biotechnology Laboratory, Biotechnology Graduate Program, University of Vale do Taquari (UNIVATES), Lajeado, Rio Grande do Sul 95914-014, Brazil., Roth G; Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 90619-900, Brazil., Chies JM; Quatro G Pesquisa & Desenvolvimento Ltd., Porto Alegre, Rio Grande do Sul 90619-900, Brazil., Yunes JA; Centro Infantil Boldrini, Campinas, São Paulo 13083-210, Brazil.; Department of Medical Genetics, Faculty of Medical Sciences, State University of Campinas, Campinas, São Paulo 13083-970, Brazil., Basso LA; National Institute of Science and Technology in Tuberculosis, Research Center for Molecular and Functional Biology, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 90619-900, Brazil.; Graduate Program in Medicine and Health Sciences, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 90619-900, Brazil.; Graduate Program in Cellular and Molecular Biology, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 90619-900, Brazil. |
المصدر: | ACS omega [ACS Omega] 2024 Aug 03; Vol. 9 (32), pp. 34951-34963. Date of Electronic Publication: 2024 Aug 03 (Print Publication: 2024). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101691658 Publication Model: eCollection Cited Medium: Internet ISSN: 2470-1343 (Electronic) Linking ISSN: 24701343 NLM ISO Abbreviation: ACS Omega Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society, [2016]- |
مستخلص: | The aims of this work were to optimize the production of Erwinia carotovora l-asparaginase II enzyme in Escherichia coli by different fed-batch cultivation strategies using a benchtop bioreactor and to evaluate the therapeutic potential of the recombinant enzyme against different acute lymphoblastic leukemia cell lines. The highest enzyme activities (∼98,000 U/L) were obtained in cultures using the DO-stat feeding strategy with induction in 18 h of culture. Under these experimental conditions, the maximum values for recombinant l-asparaginase II (rASNase) yield per substrate, rASNase yield per biomass, and productivity were approximately 1204 U/g Competing Interests: The authors declare no competing financial interest. (© 2024 The Authors. Published by American Chemical Society.) |
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تواريخ الأحداث: | Date Created: 20240819 Latest Revision: 20240820 |
رمز التحديث: | 20240820 |
مُعرف محوري في PubMed: | PMC11325515 |
DOI: | 10.1021/acsomega.4c04711 |
PMID: | 39157126 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2470-1343 |
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DOI: | 10.1021/acsomega.4c04711 |