دورية أكاديمية
Epitopes screening and vaccine molecular design of PEDV S protein based on immunoinformatics.
| العنوان: | Epitopes screening and vaccine molecular design of PEDV S protein based on immunoinformatics. |
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| المؤلفون: | Li S; ShanghaiMedicilonInc., Shanghai, 201299, China., Bai X; College of Animal Science and Technology, Shihezi University, Shihezi, 832003, China.; Xinjiang Western Animal Husbandry Co., Ltd, Shihezi, 832000, China., Wang C; Xinjiang Western Animal Husbandry Co., Ltd, Shihezi, 832000, China. wang-chaoli@163.com. |
| المصدر: | Scientific reports [Sci Rep] 2024 Aug 22; Vol. 14 (1), pp. 19537. Date of Electronic Publication: 2024 Aug 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
| مواضيع طبية MeSH: | Coronavirus Infections*/immunology , Coronavirus Infections*/prevention & control , Coronavirus Infections*/veterinary , Coronavirus Infections*/virology , Immunoinformatics*/methods , Porcine epidemic diarrhea virus*/immunology , Swine Diseases*/prevention & control , Swine Diseases*/immunology , Swine Diseases*/virology , Viral Vaccines*/immunology, Animals ; Epitopes/immunology ; Epitopes, B-Lymphocyte/immunology ; Epitopes, T-Lymphocyte/immunology ; Molecular Docking Simulation ; Spike Glycoprotein, Coronavirus/immunology ; Spike Glycoprotein, Coronavirus/chemistry ; Swine |
| مستخلص: | Porcine epidemic diarrhea virus (PEDV) is a serious disease that poses a significant threat to the pig industry. This study focused on analyzing the Spike protein of PEDV, which harbors crucial antigenic determinants, in identifying dominant epitopes. Immunoinformatics tools were used to screen for B-cell, CD4+ and CD8+ predominance epitopes. These epitopes were then connected to the N-terminal of ferritin to form a self-assembled nanoparticle vaccine. Various physical and chemical properties of the candidate vaccine were analyzed, including secondary structure prediction, tertiary structure modeling, molecular docking, immune response simulation and computer cloning. The results demonstrated that the candidate vaccine was antigenic, soluble, stable, non-allergic, and formed a stable complex with the target receptor TLR-3. Immune simulation analysis showed that the candidate vaccine effectively stimulated both cellular and humoral reactions, leading to increased related cytokines production. Furthermore, efficient and stable expression of the candidate vaccine was achieved through reverse translation in the Escherichia coli K12 expression system following codon optimization and in silico cloning. The developed nanoparticle candidate vaccine in this study holds promise as an effective PEDV vaccine candidate, offering a new approach for the research, development and improvement of vaccines targeting porcine enteric diarrhea coronavirus. (© 2024. The Author(s).) |
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| فهرسة مساهمة: | Keywords: Dominant epitope; Ferritin; Immunoinformatics; Nanoparticle vaccine; Porcine epidemic diarrhea virus; S protein |
| المشرفين على المادة: | 0 (Epitopes) 0 (Epitopes, B-Lymphocyte) 0 (Epitopes, T-Lymphocyte) 0 (Spike Glycoprotein, Coronavirus) 0 (Viral Vaccines) |
| تواريخ الأحداث: | Date Created: 20240822 Date Completed: 20240822 Latest Revision: 20240829 |
| رمز التحديث: | 20240830 |
| مُعرف محوري في PubMed: | PMC11341743 |
| DOI: | 10.1038/s41598-024-70579-0 |
| PMID: | 39174674 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 2045-2322 |
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| DOI: | 10.1038/s41598-024-70579-0 |