دورية أكاديمية
KDM6A Promotes Angiogenesis, Migration, and Invasion of Pancreatic Cancer by Activating LAMP3.
| العنوان: | KDM6A Promotes Angiogenesis, Migration, and Invasion of Pancreatic Cancer by Activating LAMP3. |
|---|---|
| المؤلفون: | Fan S; Zhejiang Hospital., Xu L; Department of General Surgery, Zhejiang Hospital, Hangzhou 310013, China., Xie D; Department of General Surgery, Zhejiang Hospital, Hangzhou, Zhejiang 310013, China., Yu B; Zhejiang Hospital. |
| المصدر: | Critical reviews in eukaryotic gene expression [Crit Rev Eukaryot Gene Expr] 2024; Vol. 34 (8), pp. 25-34. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Begell House Country of Publication: United States NLM ID: 9007261 Publication Model: Print Cited Medium: Internet ISSN: 1045-4403 (Print) Linking ISSN: 10454403 NLM ISO Abbreviation: Crit Rev Eukaryot Gene Expr Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2000- > : Reading, CT : Begell House Original Publication: Boca Raton, FL : CRC Press, 1990- |
| مواضيع طبية MeSH: | Pancreatic Neoplasms*/pathology , Pancreatic Neoplasms*/genetics , Pancreatic Neoplasms*/metabolism , Cell Movement*/genetics , Neovascularization, Pathologic*/genetics , Neovascularization, Pathologic*/metabolism , Histone Demethylases*/metabolism , Histone Demethylases*/genetics , Cell Proliferation*/genetics , Neoplasm Invasiveness* , Lysosomal Membrane Proteins*/metabolism , Lysosomal Membrane Proteins*/genetics , Gene Expression Regulation, Neoplastic*, Humans ; Cell Line, Tumor ; Angiogenesis ; Neoplasm Proteins ; Lysosomal-Associated Membrane Protein 3 |
| مستخلص: | Lysine demethylase 6A (KDM6A) is abnormally expressed in various cancer. This study aimed to investigate the potential of KDM6A in pancreatic cancer (PC). mRNA expression was calculated by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). Protein expression was detected by Western blot. Cell viability was measured by Cell Counting Kit (CCK-8) assay. Cell angiogenesis was determined by tube formation assay. Cell migration and invasion were determined by Transwell assay. We found that KDM6A was upregulated in PC patients and cells. Interestingly, KDM6A deficiency inhibited the proliferation and angiogenesis of PC cells. Moreover, KDM6A knockdown suppressed the migration and invasion of PC cells. Additionally, KDM6A upregulated the expression of lysosomal associated membrane protein 3 (LAMP3) via driving demethylation of H3K27me3. Overexpression of LAMP3 reversed the effects of KDM6A knockdown and contributed to the angiogenesis and aggressiveness of PC cells. In summary, KDM6A-mediated demethylation of tri-methylation at lysine 27 of histone H3 (H3K27me3) promotes the transcription of LAMP3, resulting the angiogenesis and aggressiveness of PC. Therefore, targeting KDM6A may be an anti-angiogenetic strategy for PC. |
| المشرفين على المادة: | EC 1.14.11.- (Histone Demethylases) EC 1.14.11.- (KDM6A protein, human) 0 (Lysosomal Membrane Proteins) 0 (LAMP3 protein, human) 0 (Neoplasm Proteins) 0 (Lysosomal-Associated Membrane Protein 3) |
| تواريخ الأحداث: | Date Created: 20240824 Date Completed: 20240824 Latest Revision: 20240824 |
| رمز التحديث: | 20240826 |
| DOI: | 10.1615/CritRevEukaryotGeneExpr.2024054038 |
| PMID: | 39180205 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1045-4403 |
|---|---|
| DOI: | 10.1615/CritRevEukaryotGeneExpr.2024054038 |