دورية أكاديمية
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Protocol for in vitro generating innate lymphoid cells from mouse α 4 β 7 + lymphoid progenitors.

التفاصيل البيبلوغرافية
العنوان: Protocol for in vitro generating innate lymphoid cells from mouse α 4 β 7 + lymphoid progenitors.
المؤلفون: Wu T; School of Medicine, Institute for Immunology, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Center for Life Science, Beijing, China. Electronic address: wutao13@tsinghua.org.cn., Wang Y; School of Medicine, Institute for Immunology, Tsinghua University, Beijing 100084, China., Wu L; School of Medicine, Institute for Immunology, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Center for Life Science, Beijing, China; Beijing Key Laboratory for Immunological Research on Chronic Diseases, Beijing 100084, China. Electronic address: wuli@tsinghua.edu.cn.
المصدر: STAR protocols [STAR Protoc] 2024 Sep 20; Vol. 5 (3), pp. 103229. Date of Electronic Publication: 2024 Aug 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Cambridge, MA] : Cell Press, [2020]-
مواضيع طبية MeSH: Cell Differentiation* , Flow Cytometry*/methods , Immunity, Innate* , Lymphocytes*/cytology , Lymphocytes*/immunology, Animals ; Mice ; Lymphoid Progenitor Cells/cytology ; Lymphoid Progenitor Cells/metabolism ; Cell Culture Techniques/methods ; Cells, Cultured ; Cell Separation/methods ; Integrins
مستخلص: Building a simple and efficient in vitro differentiation system is crucial for studying the regulatory mechanisms during the development of innate lymphoid cells (ILCs). Here, we present a protocol for generating ILC subsets from α 4 β 7 + lymphoid progenitors (αLPs). We describe steps for murine cell isolation from fetal liver and adult bone marrow, flow cytometry sorting for αLPs, and cell culture. We then detail procedures for flow cytometry analysis of ILCs. This protocol significantly simplifies the differentiation process through ILC differentiation in vitro. For complete details on the use and execution of this protocol, please refer to Wu et al. 1 .
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: cell differentiation; developmental biology; immunology
المشرفين على المادة: 0 (integrin alpha4beta7)
0 (Integrins)
تواريخ الأحداث: Date Created: 20240824 Date Completed: 20240926 Latest Revision: 20240926
رمز التحديث: 20240926
مُعرف محوري في PubMed: PMC11388591
DOI: 10.1016/j.xpro.2024.103229
PMID: 39180747
قاعدة البيانات: MEDLINE
الوصف
تدمد:2666-1667
DOI:10.1016/j.xpro.2024.103229