دورية أكاديمية
أعرض في EDS

Ultrasound-mediated spatial and temporal control of engineered cells in vivo.

التفاصيل البيبلوغرافية
العنوان: Ultrasound-mediated spatial and temporal control of engineered cells in vivo.
المؤلفون: Ivanovski F; Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, Ljubljana, Slovenia.; Interfaculty Doctoral Study of Biomedicine, University of Ljubljana, Vrazov trg 2, Ljubljana, Slovenia., Meško M; Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, Ljubljana, Slovenia., Lebar T; Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, Ljubljana, Slovenia., Rupnik M; Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, Ljubljana, Slovenia., Lainšček D; Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, Ljubljana, Slovenia., Gradišek M; Faculty of Electrical Engineering, University of Ljubljana, Tržaška c. 25, Ljubljana, Slovenia., Jerala R; Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, Ljubljana, Slovenia. roman.jerala@ki.si.; CTGCT, Centre of Technology of Gene and Cell Therapy, Hajdrihova 19, Ljubljana, Slovenia. roman.jerala@ki.si., Benčina M; Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, Ljubljana, Slovenia. mojca.bencina@ki.si.; CTGCT, Centre of Technology of Gene and Cell Therapy, Hajdrihova 19, Ljubljana, Slovenia. mojca.bencina@ki.si.; University of Ljubljana, Kongresni trg 12, 1000, Ljubljana, Slovenia. mojca.bencina@ki.si.
المصدر: Nature communications [Nat Commun] 2024 Aug 27; Vol. 15 (1), pp. 7369. Date of Electronic Publication: 2024 Aug 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Colitis*/pathology , Colitis*/therapy , Ultrasonic Waves*, Animals ; Humans ; HEK293 Cells ; Mice ; Calcium/metabolism ; Cell Engineering/methods ; Mice, Inbred C57BL ; Female ; Transcription Factors/metabolism ; Transcription Factors/genetics
مستخلص: Remote regulation of cells in deep tissue remains a significant challenge. Low-intensity pulsed ultrasound offers promise for in vivo therapies due to its non-invasive nature and precise control. This study uses pulsed ultrasound to control calcium influx in mammalian cells and engineers a therapeutic cellular device responsive to acoustic stimulation in deep tissue without overexpressing calcium channels or gas vesicles. Pulsed ultrasound parameters are established to induce calcium influx in HEK293 cells. Additionally, cells are engineered to express a designed calcium-responsive transcription factor controlling the expression of a selected therapeutic gene, constituting a therapeutic cellular device. The engineered sonogenetic system's functionality is demonstrated in vivo in mice, where an implanted anti-inflammatory cytokine-producing cellular device effectively alleviates acute colitis, as shown by improved colonic morphology and histopathology. This approach provides a powerful tool for precise, localized control of engineered cells in deep tissue, showcasing its potential for targeted therapeutic delivery.
(© 2024. The Author(s).)
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المشرفين على المادة: SY7Q814VUP (Calcium)
0 (Transcription Factors)
تواريخ الأحداث: Date Created: 20240827 Date Completed: 20240827 Latest Revision: 20240830
رمز التحديث: 20240830
مُعرف محوري في PubMed: PMC11349879
DOI: 10.1038/s41467-024-51620-2
PMID: 39191796
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-024-51620-2