دورية أكاديمية
Preparation of a Lactobacillus rhamnosus ATCC 7469 microencapsulated-lactulose synbiotic and its effect on equol production.
| العنوان: | Preparation of a Lactobacillus rhamnosus ATCC 7469 microencapsulated-lactulose synbiotic and its effect on equol production. |
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| المؤلفون: | Wang X; College of Food Science, South China Agricultural University, Guangzhou, 510642, China. 56809509@qq.com., Ma Y; College of Food Science, South China Agricultural University, Guangzhou, 510642, China. 56809509@qq.com., Liu Y; College of Food Science, South China Agricultural University, Guangzhou, 510642, China. 56809509@qq.com., Zhang J; College of Food Science, South China Agricultural University, Guangzhou, 510642, China. 56809509@qq.com., Jiang W; College of Food Science, South China Agricultural University, Guangzhou, 510642, China. 56809509@qq.com., Fang X; College of Food Science, South China Agricultural University, Guangzhou, 510642, China. 56809509@qq.com., Wang L; College of Food Science, South China Agricultural University, Guangzhou, 510642, China. 56809509@qq.com. |
| المصدر: | Food & function [Food Funct] 2024 Sep 16; Vol. 15 (18), pp. 9471-9487. Date of Electronic Publication: 2024 Sep 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101549033 Publication Model: Electronic Cited Medium: Internet ISSN: 2042-650X (Electronic) Linking ISSN: 20426496 NLM ISO Abbreviation: Food Funct Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge : Royal Society of Chemistry |
| مواضيع طبية MeSH: | Lacticaseibacillus rhamnosus*/metabolism , Synbiotics* , Equol* , Probiotics* , Lactulose*/metabolism, Humans ; Alginates/chemistry ; Drug Compounding/methods ; Chitosan/chemistry ; Colon/microbiology ; Colon/metabolism |
| مستخلص: | Equol is a highly active product of soy isoflavones produced by specific bacteria in the human or animal colon. However, equol production is influenced by differences in the gut flora carried by the body. Our previous research has shown that a synbiotic preparation comprising the probiotic Lactobacillus rhamnosus ATCC 7469 and the prebiotic lactulose can enhance equol production by modulating the intestinal flora. Nevertheless, the harsh environment of the gastrointestinal tract limits this capability by diminishing the number of probiotics reaching the colon. Microencapsulation of probiotics is an effective strategy to enhance their viability. In this study, probiotic gel microspheres (SA-S-CS) were prepared using an extrusion method, with sodium alginate (SA) and chitosan (CS) serving as the encapsulating materials. Scanning electron microscopy (SEM) was employed to observe the surface morphology and the internal distribution of bacteria within the microcapsules. The structural characteristics of the microcapsules were investigated using Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). Furthermore, the thermal stability, storage stability, probiotic viability post-simulated gastrointestinal fluid treatment, and colon release rate were examined. Finally, the impact of probiotic microencapsulation on promoting equol production by the synbiotic preparation was assessed. The results indicated that the microcapsules exhibited a spherical structure with bacteria evenly distributed on the inner surface. Studies on thermal and storage stability showed that the number of viable cells in the probiotic microcapsule group significantly increased compared to the free probiotic group. Gastrointestinal tolerance studies revealed that after in vitro simulated gastrointestinal digestion, the amount of viable cells in the microcapsules was 7 log |
| المشرفين على المادة: | 531-95-3 (Equol) 4618-18-2 (Lactulose) 0 (Alginates) 9012-76-4 (Chitosan) |
| تواريخ الأحداث: | Date Created: 20240828 Date Completed: 20240916 Latest Revision: 20240916 |
| رمز التحديث: | 20240917 |
| DOI: | 10.1039/d4fo02690j |
| PMID: | 39193624 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2042-650X |
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| DOI: | 10.1039/d4fo02690j |