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Exploring the Mutated Kinases for Chemoenzymatic Synthesis of N 4 -Modified Cytidine Monophosphates.

التفاصيل البيبلوغرافية
العنوان: Exploring the Mutated Kinases for Chemoenzymatic Synthesis of N 4 -Modified Cytidine Monophosphates.
المؤلفون: Koplūnaitė M; Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio Av. 7, LT-10257 Vilnius, Lithuania., Butkutė K; Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio Av. 7, LT-10257 Vilnius, Lithuania., Stankevičiūtė J; Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio Av. 7, LT-10257 Vilnius, Lithuania., Meškys R; Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio Av. 7, LT-10257 Vilnius, Lithuania.
المصدر: Molecules (Basel, Switzerland) [Molecules] 2024 Aug 09; Vol. 29 (16). Date of Electronic Publication: 2024 Aug 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, c1995-
مواضيع طبية MeSH: Drosophila melanogaster*/enzymology , Drosophila melanogaster*/genetics , Cytidine Monophosphate*/analogs & derivatives , Cytidine Monophosphate*/metabolism , Cytidine Monophosphate*/chemistry, Animals ; Phosphorylation ; Substrate Specificity ; Phosphotransferases/genetics ; Phosphotransferases/metabolism ; Phosphotransferases/chemistry ; Bacillus subtilis/enzymology ; Bacillus subtilis/genetics ; Mutation ; Deoxycytidine Kinase/genetics ; Deoxycytidine Kinase/metabolism ; Deoxycytidine Kinase/chemistry
مستخلص: Nucleosides, nucleotides, and their analogues are an important class of molecules that are used as substrates in research of enzymes and nucleic acid, or as antiviral and antineoplastic agents. Nucleoside phosphorylation is usually achieved with chemical methods; however, enzymatic phosphorylation is a viable alternative. Here, we present a chemoenzymatic synthesis of modified cytidine monophosphates, where a chemical synthesis of novel N 4 -modified cytidines is followed by an enzymatic phosphorylation of the nucleosides by nucleoside kinases. To enlarge the substrate scope, multiple mutant variants of Drosophila melanogaster deoxynucleoside kinase ( Dm dNK) (EC:2.7.1.145) and Bacillus subtilis deoxycytidine kinase ( Bs dCK) (EC:2.7.1.74) have been created and tested. It has been determined that certain point mutations in the active sites of the kinases alter their substrate specificities noticeably and allow phosphorylation of compounds that had been otherwise not phosphorylated by the wild-type Dm dNK or Bs dCK.
References: FEBS J. 2008 May;275(9):2151-60. (PMID: 18384378)
Molecules. 2020 Apr 28;25(9):. (PMID: 32354007)
Front Bioeng Biotechnol. 2020 Aug 06;8:854. (PMID: 32903716)
J Bioenerg Biomembr. 2000 Jun;32(3):237-46. (PMID: 11768307)
Biochemistry. 2003 May 20;42(19):5706-12. (PMID: 12741827)
J Biol Chem. 2000 Mar 3;275(9):6673-9. (PMID: 10692477)
Curr Gene Ther. 2003 Feb;3(1):13-26. (PMID: 12553532)
EMBO J. 2002 Apr 2;21(7):1873-80. (PMID: 11927571)
ACS Synth Biol. 2017 Mar 17;6(3):388-394. (PMID: 27935283)
FEBS J. 2007 Mar;274(6):1542-51. (PMID: 17302737)
Nat Methods. 2020 Mar;17(3):311-318. (PMID: 32015544)
Science. 2022 Nov 25;378(6622):899-904. (PMID: 36423275)
Antiviral Res. 2010 Apr;86(1):101-20. (PMID: 20417378)
Biochem Biophys Res Commun. 2009 May 1;382(2):430-3. (PMID: 19285960)
Nucleosides Nucleotides Nucleic Acids. 2009 Aug;28(8):776-92. (PMID: 20183617)
J Biol Chem. 1999 Aug 20;274(34):23814-9. (PMID: 10446143)
Nat Methods. 2022 Jun;19(6):679-682. (PMID: 35637307)
Gene Ther. 2007 Sep;14(17):1278-86. (PMID: 17581598)
J Mol Biol. 2000 Aug 25;301(4):827-37. (PMID: 10966789)
Sci Adv. 2023 Feb 3;9(5):eade4361. (PMID: 36735785)
Eur J Biochem. 2003 Jul;270(13):2879-84. (PMID: 12823558)
ACS Synth Biol. 2023 Jun 16;12(6):1772-1781. (PMID: 37227319)
Curr Gene Ther. 2019;19(1):54-65. (PMID: 30848201)
Appl Microbiol Biotechnol. 2013 Nov;97(21):9389-95. (PMID: 23974370)
Chem Rev. 2016 Jul 27;116(14):7854-97. (PMID: 27319940)
Gene Ther. 2007 Jan;14(1):86-92. (PMID: 16885999)
Pharmacol Ther. 2000 Aug-Sep;87(2-3):189-98. (PMID: 11008000)
Trends Biochem Sci. 2005 May;30(5):225-8. (PMID: 15896737)
J Am Chem Soc. 2002 Dec 11;124(49):14626-30. (PMID: 12465973)
PLoS One. 2017 Mar 21;12(3):e0174163. (PMID: 28323896)
J Biol Chem. 2001 Feb 23;276(8):5518-24. (PMID: 11078735)
Nat Struct Biol. 2001 Jul;8(7):616-20. (PMID: 11427893)
J Am Chem Soc. 2020 Aug 26;142(34):14417-14421. (PMID: 32786764)
ACS Omega. 2024 Feb 14;9(8):9300-9308. (PMID: 38434802)
J Gene Med. 2011 Jun;13(6):305-11. (PMID: 21674733)
Mol Biol. 2021;55(6):786-812. (PMID: 34955556)
Protein Sci. 1997 Oct;6(10):2097-106. (PMID: 9336833)
J Bacteriol. 1995 Sep;177(17):4921-6. (PMID: 7665468)
Mol Pharmacol. 2001 May;59(5):1181-6. (PMID: 11306702)
J Biol Chem. 1980 Sep 10;255(17):8216-20. (PMID: 6251049)
Methods Mol Biol. 2017;1498:375-383. (PMID: 27709590)
Pharmacol Rev. 2013 Apr 16;65(3):906-43. (PMID: 23592612)
J Genet Genomics. 2015 May 20;42(5):235-48. (PMID: 26059771)
معلومات مُعتمدة: S-MIP-22-6 Lietuvos Mokslo Taryba
فهرسة مساهمة: Keywords: biocatalysis; chemical synthesis; deoxycytidine kinase; deoxynucleoside kinase; mutagenesis; nucleoside 5′-monophosphates; nucleoside analogues; nucleotides
المشرفين على المادة: F469818O25 (Cytidine Monophosphate)
EC 2.7.- (Phosphotransferases)
EC 2.7.1.74 (Deoxycytidine Kinase)
EC 2.7.1.77 (nucleoside phosphotransferase)
تواريخ الأحداث: Date Created: 20240829 Date Completed: 20240829 Latest Revision: 20240901
رمز التحديث: 20240902
مُعرف محوري في PubMed: PMC11357392
DOI: 10.3390/molecules29163767
PMID: 39202847
قاعدة البيانات: MEDLINE
الوصف
تدمد:1420-3049
DOI:10.3390/molecules29163767