دورية أكاديمية
أعرض في EDS

HIV-1 residual risk and pre-treatment drug resistance among blood donors: A sentinel surveillance from Gabon.

التفاصيل البيبلوغرافية
العنوان: HIV-1 residual risk and pre-treatment drug resistance among blood donors: A sentinel surveillance from Gabon.
المؤلفون: Mangala C; Chantal BIYA International Reference Centre for research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; School of Health Sciences, Catholic University of Central Africa (ESS-UCAC), Yaoundé, Cameroon.; Virology Department, National Public Health Laboratory (NPHL), Libreville, Gabon., Takou D; Chantal BIYA International Reference Centre for research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Maulot-Bangola D; Chantal BIYA International Reference Centre for research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; School of Health Sciences, Catholic University of Central Africa (ESS-UCAC), Yaoundé, Cameroon.; Virology Department, National Blood Transfusion Centre (NBTC), Libreville, Gabon., Beloumou G; Chantal BIYA International Reference Centre for research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Rebienot Pellegrin O; Virology Department, National Blood Transfusion Centre (NBTC), Libreville, Gabon., Sosso SM; Chantal BIYA International Reference Centre for research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Ambe Chenwi C; Chantal BIYA International Reference Centre for research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; Faculty of Medicine and Surgery, University of Rome Tor Vergata, Rome, Italy., Ngoufack Jagni Semengue E; Chantal BIYA International Reference Centre for research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Vigan Codjo F; Virology Department, National Blood Transfusion Centre (NBTC), Libreville, Gabon., Boussougou O; Virology Department, National Blood Transfusion Centre (NBTC), Libreville, Gabon., Nka AD; Chantal BIYA International Reference Centre for research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Tommo M; Chantal BIYA International Reference Centre for research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; School of Health Sciences, Catholic University of Central Africa (ESS-UCAC), Yaoundé, Cameroon., Fainguem N; Chantal BIYA International Reference Centre for research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Kamgaing R; Chantal BIYA International Reference Centre for research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon., Ama Moor V; School of Health Sciences, Catholic University of Central Africa (ESS-UCAC), Yaoundé, Cameroon.; Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1 (FMBS-UY1), Yaoundé, Cameroon.; Laboratories of Biochemistry and Microbiology, University Teaching Hospital, Yaoundé, Cameroon., Kamga Gonsu H; School of Health Sciences, Catholic University of Central Africa (ESS-UCAC), Yaoundé, Cameroon.; Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1 (FMBS-UY1), Yaoundé, Cameroon.; Laboratories of Biochemistry and Microbiology, University Teaching Hospital, Yaoundé, Cameroon., Penlap V; School of Health Sciences, Catholic University of Central Africa (ESS-UCAC), Yaoundé, Cameroon.; Biotechnology Centre, University of Yaounde I, Yaounde, Cameroon., Nkoa T; School of Health Sciences, Catholic University of Central Africa (ESS-UCAC), Yaoundé, Cameroon.; Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1 (FMBS-UY1), Yaoundé, Cameroon., Colizzi V; Chantal BIYA International Reference Centre for research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; Faculty of Science and Technology, Evangelic University of Cameroon, Bandjoun, Cameroon.; UNESCO Chair of Biotechnology, University of Rome Tor Vergata, Rome, Italy., Perno CF; Chantal BIYA International Reference Centre for research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; Department of Microbiology, Bambino Gesu Pediatric Hospital, Rome, Italy., Fokam J; Chantal BIYA International Reference Centre for research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; School of Health Sciences, Catholic University of Central Africa (ESS-UCAC), Yaoundé, Cameroon.; Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1 (FMBS-UY1), Yaoundé, Cameroon.; Faculty of Health Sciences, University of Buea (FHS-UB), Buea, Cameroon.; National HIV Drug Resistance working group, Ministry of Public Health, Yaounde, Cameroon., Ndjolo A; Chantal BIYA International Reference Centre for research on HIV/AIDS Prevention and Management (CIRCB), Yaoundé, Cameroon.; Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1 (FMBS-UY1), Yaoundé, Cameroon.
المصدر: PloS one [PLoS One] 2024 Sep 03; Vol. 19 (9), pp. e0305935. Date of Electronic Publication: 2024 Sep 03 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Blood Donors*/statistics & numerical data , HIV-1*/genetics , HIV-1*/drug effects , HIV Infections*/epidemiology , HIV Infections*/virology , HIV Infections*/drug therapy , HIV Infections*/transmission , Drug Resistance, Viral*/genetics , Sentinel Surveillance*, Humans ; Gabon/epidemiology ; Male ; Female ; Adult ; Middle Aged ; Viral Load ; Phylogeny ; Young Adult ; Adolescent ; Anti-HIV Agents/therapeutic use ; Anti-HIV Agents/pharmacology
مستخلص: Background: Surveillance of HIV-1 pre-treatment drug resistance (PDR) is essential for ensuring the success of first-line antiretroviral therapy (ART). Beside population-based surveys, sentinel surveillance of PDR and circulating HIV-1 clades in specific populations such as blood donors could efficiently inform decision-making on ART program. We therefore sought to ascertain HIV-1 residual infection, the threshold of PDR and viral diversity among recently-diagnosed blood donors in Gabon.
Methods: A sentinel surveillance was conducted among 381 consenting blood donors at the National Blood Transfusion Center (NBTC) in Gabon from August 3,2020 to August, 31, 2021. In order to determine the residual risk of HIV transmission, viral load and HIV-1 Sanger-sequencing were performed at the Chantal BIYA International Reference Center (CIRCB)-Cameroon on HIV samples previously tested seronegative with ELISA in Gabon. Phylogeny was performed using MEGA X, PDR threshold>10% was considered high and data were analysed using p≤0.05 for statistical significance.
Results: Five HIV-negative blood donors had a detectable viral load indicating a high residual risk of HIV transmission. Among the samples successfully sequenced, four participants had major drug resistance mutations (DRMs), giving a threshold of PDR of 25% (4/16). By drug class, major DRMs targeting NNRTI (K103N, E138G), NRTIs (L210W) and PI/r (M46L). The most representative viral clades were CRF02_AG and subtype A1. The genetic diversity of HIV-1 had no significant effect on the residual risk in blood transfusion (CRF02_AG, P = 0.3 and Recombinants, P = 0.5).
Conclusion: This sentinel surveillance indicates a high residual risk of HIV-1 transfusion in Gabon, thereby underscoring the need for optimal screening strategy for blood safety. Moreover, HIV-1 transmission goes with high-risk of PDR, suggesting suboptimal efficacy of ART. Nonetheless, the genetic diversity has limited (if any effect) on the residual risk of infection and PDR in blood donors.
Competing Interests: The authors have declared that there are no interests competitors.
(Copyright: © 2024 Mangala et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
المشرفين على المادة: 0 (Anti-HIV Agents)
تواريخ الأحداث: Date Created: 20240903 Date Completed: 20240903 Latest Revision: 20240903
رمز التحديث: 20240904
DOI: 10.1371/journal.pone.0305935
PMID: 39226273
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0305935