دورية أكاديمية
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Genetic diversity analysis and DNA fingerprint construction of Zanthoxylum species based on SSR and iPBS markers.

التفاصيل البيبلوغرافية
العنوان: Genetic diversity analysis and DNA fingerprint construction of Zanthoxylum species based on SSR and iPBS markers.
المؤلفون: Zhang X; College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei, 434025, China., Chen W; Sichuan Academy of Forestry, Chengdu, Sichuan, 610081, China., Yang Z; Sichuan Academy of Forestry, Chengdu, Sichuan, 610081, China., Luo C; Sichuan Academy of Forestry, Chengdu, Sichuan, 610081, China., Zhang W; College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei, 434025, China. wwzhangchn@163.com., Xu F; College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei, 434025, China. xufeng@yangtzeu.edu.cn., Ye J; College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei, 434025, China., Liao Y; College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei, 434025, China.
المصدر: BMC plant biology [BMC Plant Biol] 2024 Sep 07; Vol. 24 (1), pp. 843. Date of Electronic Publication: 2024 Sep 07.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Zanthoxylum*/genetics , Microsatellite Repeats*/genetics , Genetic Variation* , DNA Fingerprinting*, Genetic Markers ; Phylogeny ; DNA, Plant/genetics ; Polymorphism, Genetic ; Alleles ; Binding Sites
مستخلص: Zanthoxylum is a versatile economic tree species utilized for its spice, seasoning, oil, medicinal, and industrial raw material applications, and it has a lengthy history of cultivation and domestication in China. This has led to the development of numerous cultivars. However, the phenomenon of mixed cultivars and confusing names has significantly obstructed the effective utilization of Zanthoxylum resources and industrial development. Consequently, conducting genetic diversity studies and cultivar identification on Zanthoxylum are crucial. This research analyzed the genetic traits of 80 Zanthoxylum cultivars using simple sequence repeat (SSR) and inter-Primer Binding Site (iPBS) molecular markers, leading to the creation of a DNA fingerprint. This study identified 206 and 127 alleles with 32 SSR markers and 10 iPBS markers, respectively, yielding an average of 6.4 and 12.7 alleles (Na) per marker. The average polymorphism information content (PIC) for the SSR and iPBS markers was 0.710 and 0.281, respectively. The genetic similarity coefficients for the 80 Zanthoxylum accessions ranged from 0.0947 to 0.9868 and from 0.2206 to 1.0000, with mean values of 0.3864 and 0.5215, respectively, indicating substantial genetic diversity. Cluster analysis, corroborated by principal coordinate analysis (PCoA), categorized these accessions into three primary groups. Analysis of the genetic differentiation among the three Zanthoxylum (Z. bungeanum, Z. armatum, and Z. piperitum) populations using SSR markers revealed a mean genetic differentiation coefficient (Fst) of 0.335 and a gene flow (Nm) of 0.629, suggesting significant genetic divergence among the populations. Molecular variance analysis (AMOVA) indicated that 65% of the genetic variation occurred within individuals, while 35% occurred among populations. Bayesian model-based analysis of population genetic structure divided all materials into two groups. The combined PI and PIsibs value of the 32 SSR markers were 4.265 × 10 - 27 and 1.282 × 10 - 11 , respectively, showing strong fingerprinting power. DNA fingerprints of the 80 cultivars were established using eight pairs of SSR primers, each assigned a unique numerical code. In summary, while both markers were effective at assessing the genetic diversity and relationships of Zanthoxylum species, SSR markers demonstrated superior polymorphism and cultivar discrimination compared to iPBS markers. These findings offer a scientific foundation for the conservation and sustainable use of Zanthoxylum species.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 2019YFD1001200 National Key R&D Program of China; 2019YFD1001200 National Key R&D Program of China; 2019YFD1001200 National Key R&D Program of China; 2019YFD1001200 National Key R&D Program of China; 2019YFD1001200 National Key R&D Program of China; 2021YFYZ0032 Research on the Selection and Breeding of New High-Quality and Labor-Saving Cultivars of Chinese Pepper and Supporting Technology; 2021YFYZ0032 Research on the Selection and Breeding of New High-Quality and Labor-Saving Cultivars of Chinese Pepper and Supporting Technology; 2021YFYZ0032 Research on the Selection and Breeding of New High-Quality and Labor-Saving Cultivars of Chinese Pepper and Supporting Technology
فهرسة مساهمة: Keywords: Zanthoxylum; DNA fingerprint; Genetic diversity; Genetic structure; SSR markers; iPBS markers
المشرفين على المادة: 0 (Genetic Markers)
0 (DNA, Plant)
تواريخ الأحداث: Date Created: 20240907 Date Completed: 20240907 Latest Revision: 20240910
رمز التحديث: 20240910
مُعرف محوري في PubMed: PMC11380355
DOI: 10.1186/s12870-024-05373-1
PMID: 39244564
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2229
DOI:10.1186/s12870-024-05373-1