دورية أكاديمية
أعرض في EDS

Simple supplementation of serum-free medium produces gametocytes of Plasmodium falciparum that transmit to mosquitoes.

التفاصيل البيبلوغرافية
العنوان: Simple supplementation of serum-free medium produces gametocytes of Plasmodium falciparum that transmit to mosquitoes.
المؤلفون: Pradhan S; School of Biodiversity, One Health and Veterinary Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK. sabyasachi.pradhan@glasgow.ac.uk., Ubiaru PC; School of Biodiversity, One Health and Veterinary Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK., Ranford-Cartwright L; School of Biodiversity, One Health and Veterinary Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK. Lisa.ranford-cartwright@glasgow.ac.uk.
المصدر: Malaria journal [Malar J] 2024 Sep 10; Vol. 23 (1), pp. 275. Date of Electronic Publication: 2024 Sep 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101139802 Publication Model: Electronic Cited Medium: Internet ISSN: 1475-2875 (Electronic) Linking ISSN: 14752875 NLM ISO Abbreviation: Malar J Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2002-
مواضيع طبية MeSH: Plasmodium falciparum*/physiology , Plasmodium falciparum*/drug effects , Plasmodium falciparum*/growth & development , Anopheles*/parasitology, Animals ; Culture Media, Serum-Free ; Humans ; Malaria, Falciparum/parasitology ; Malaria, Falciparum/prevention & control
مستخلص: Background: Human serum is a major component of Plasmodium falciparum culture medium, and can be replaced with AlbuMAX ™ II, a lipid-rich bovine serum albumin, for asexual cultures. However, gametocytes produced without serum are poorly infective to mosquitoes. Serum suffers from high cost, limited availability, and variability in quality.
Methods: Several commercially-available media supplements were tested for their ability to support parasite growth and production of P. falciparum (3D7) gametocytes in standard RPMI1640 medium containing 0.5% AlbuMAX. The impact on asexual growth and gametocyte production with each supplement was assessed and compared to standard RPMI1640 medium containing 10% human serum, as well as to medium containing 0.5% AlbuMAX alone. The infectivity of gametocytes produced with one supplement to Anopheles gambiae sensu stricto was assessed by standard membrane feeding assay and measuring both prevalence of infection and oocyst intensity.
Results: Supplementation of medium containing 0.5% AlbuMAX with five supplements did not affect asexual growth of P. falciparum, and four of the five supplements supported early gametocyte production. The supplement producing the highest number of gametocytes, ITS-X, was further investigated and was found to support the production of mature gametocytes. Infection prevalence and oocyst intensity did not differ significantly between mosquitoes given a membrane feed containing gametocytes grown in medium with 0.5% AlbuMAX + ITS-X and those grown in medium with 10% human serum. Infection prevalence and oocyst intensity was significantly higher in case of ITS-X supplementation when compared to AlbuMAX alone. Infectious gametocytes were also produced from two field clones using ITS-X supplementation.
Conclusions: Serum-free medium supplemented with ITS-X was able to support the growth of gametocytes of P. falciparum that were as infectious to An. gambiae as those grown in medium with 10% serum. This is the first fully serum-free culture system able to produce highly infectious gametocytes, thereby removing the requirement for access to serum for transmission assays.
(© 2024. The Author(s).)
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معلومات مُعتمدة: S22-08 Tenovus; S22-08 Tenovus
فهرسة مساهمة: Keywords: Plasmodium falciparum; AlbuMAX™; Culture; Gametocyte; Serum; Transmission
المشرفين على المادة: 0 (Culture Media, Serum-Free)
تواريخ الأحداث: Date Created: 20240910 Date Completed: 20240911 Latest Revision: 20240914
رمز التحديث: 20240914
مُعرف محوري في PubMed: PMC11389287
DOI: 10.1186/s12936-024-05094-8
PMID: 39256807
قاعدة البيانات: MEDLINE
الوصف
تدمد:1475-2875
DOI:10.1186/s12936-024-05094-8