دورية أكاديمية
أعرض في EDS

G-Protein-Coupled Receptor Kinase 2 Inhibition Induces Meiotic Arrest by Disturbing Ca 2+ Release in Porcine Oocytes.

التفاصيل البيبلوغرافية
العنوان: G-Protein-Coupled Receptor Kinase 2 Inhibition Induces Meiotic Arrest by Disturbing Ca 2+ Release in Porcine Oocytes.
المؤلفون: Kim JD; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Lee SH; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Li XH; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Lu QY; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Zhan CL; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Lee GH; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Sim JM; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Song HJ; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Zhou D; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Cui XS; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea.
المصدر: Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2024 Sep; Vol. 59 (9), pp. e14715.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Paul Parey Scientific Publishers Country of Publication: Germany NLM ID: 9015668 Publication Model: Print Cited Medium: Internet ISSN: 1439-0531 (Electronic) Linking ISSN: 09366768 NLM ISO Abbreviation: Reprod Domest Anim Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin ; Hamburg : Paul Parey Scientific Publishers, c1990-
مواضيع طبية MeSH: Oocytes*/drug effects , Meiosis*/drug effects , G-Protein-Coupled Receptor Kinase 2*/metabolism , Calcium*/metabolism, Animals ; Female ; Swine ; Maturation-Promoting Factor/metabolism ; In Vitro Oocyte Maturation Techniques/veterinary
مستخلص: G-protein-coupled receptor kinase 2 (GRK2) interacts with Gβγ and Gαq, subunits of G-protein alpha, to regulate cell signalling. The second messenger inositol trisphosphate, produced by activated Gαq, promotes calcium release from the endoplasmic reticulum (ER) and regulates maturation-promoting factor (MPF) activity. This study aimed to investigate the role of GRK2 in MPF activity during the meiotic maturation of porcine oocytes. A specific inhibitor of GRK2 (βi) was used in this study. The present study showed that GRK2 inhibition increased the percentage of oocyte arrest at the metaphase I (MI) stage (control: 13.84 ± 0.95%; βi: 31.30 ± 4.18%), which resulted in the reduction of the maturation rate (control: 80.36 ± 1.94%; βi: 65.40 ± 1.14%). The level of phospho-GRK2 decreased in the treated group, suggesting that GRK2 activity was reduced upon GRK2 inhibition. Furthermore, the addition of βi decreased Ca 2+ release from the ER. The protein levels of cyclin B and cyclin-dependent kinase 1 were higher in the treatment group than those in the control group, indicating that GRK2 inhibition prevented a decrease in MPF activity. Collectively, GRK2 inhibition induced meiotic arrest at the MI stage in porcine oocytes by preventing a decrease in MPF activity, suggesting that GRK2 is essential for oocyte meiotic maturation in pigs.
(© 2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.)
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معلومات مُعتمدة: 2022R1A2C300769 National Research Foundation of Korea
فهرسة مساهمة: Keywords: Ca2+; GRK2; Gαq; MPF; meiotic maturation; oocyte maturation
المشرفين على المادة: EC 2.7.11.16 (G-Protein-Coupled Receptor Kinase 2)
SY7Q814VUP (Calcium)
EC 2.7.11.22 (Maturation-Promoting Factor)
تواريخ الأحداث: Date Created: 20240912 Date Completed: 20240912 Latest Revision: 20240912
رمز التحديث: 20240912
DOI: 10.1111/rda.14715
PMID: 39262106
قاعدة البيانات: MEDLINE
الوصف
تدمد:1439-0531
DOI:10.1111/rda.14715