دورية أكاديمية
TRPM4 inhibition slows neuritogenesis progression of cortical neurons.
| العنوان: | TRPM4 inhibition slows neuritogenesis progression of cortical neurons. |
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| المؤلفون: | Riquelme D; Department of Biology, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago, Chile., Juanchuto-Viertel N; Department of Biology, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago, Chile., Álamos C; Department of Biology, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago, Chile., Leiva-Salcedo E; Department of Biology, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago, Chile. elias.leiva@usach.cl. |
| المصدر: | Molecular brain [Mol Brain] 2024 Sep 12; Vol. 17 (1), pp. 66. Date of Electronic Publication: 2024 Sep 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101468876 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-6606 (Electronic) Linking ISSN: 17566606 NLM ISO Abbreviation: Mol Brain Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : BioMed Central |
| مواضيع طبية MeSH: | TRPM Cation Channels*/metabolism , TRPM Cation Channels*/antagonists & inhibitors , Neurites*/metabolism , Neurites*/drug effects , Neurogenesis* , Calcium*/metabolism , Cerebral Cortex*/cytology , Cerebral Cortex*/metabolism, Animals ; Neurons/metabolism |
| مستخلص: | TRPM4 is a non-selective cation channel activated by intracellular Ca 2+ but only permeable to monovalent cations, its activation regulates membrane potential and intracellular calcium. This channel participates in the migration and adhesion of non-excitable cells and forms an integral part of the focal adhesion complex. In neurons, TRPM4 expression starts before birth and its function at this stage is not clear, but it may function in processes such as neurite development. Here we investigate the role of TRPM4 in neuritogenesis. We found that neurons at DIV 0 express TRPM4, the inhibition of TRPM4 using 9-Ph reduces neurite number and slows the progression of neurite development, keeping neurons in stage 1. The genetic suppression of TRPM4 using an shRNA at later stages (DIV2) reduces neurite length. Conversely, at DIV 0, TRPM4 inhibition augments the Cch-induced Ca 2 + (© 2024. The Author(s).) |
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| معلومات مُعتمدة: | FONDECYT 1220680 Agencia Nacional de Investigación y Desarrollo; FONDECYT 11180536 Agencia Nacional de Investigación y Desarrollo; Proyecto Postdoc DICYT, codigo 022243LS_Postdoc Vicerrectoría de Investigación, Desarrollo e Innovación |
| فهرسة مساهمة: | Keywords: Cortical neuron development; Intracellular calcium; Neuritogenesis; TRPM4 |
| المشرفين على المادة: | 0 (TRPM Cation Channels) SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 20240912 Date Completed: 20240913 Latest Revision: 20240915 |
| رمز التحديث: | 20240915 |
| مُعرف محوري في PubMed: | PMC11391768 |
| DOI: | 10.1186/s13041-024-01140-3 |
| PMID: | 39267102 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1756-6606 |
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| DOI: | 10.1186/s13041-024-01140-3 |