دورية أكاديمية
Near-infrared in vivo imaging system for dynamic visualization of lung-colonizing bacteria in mouse pneumonia.
| العنوان: | Near-infrared in vivo imaging system for dynamic visualization of lung-colonizing bacteria in mouse pneumonia. |
|---|---|
| المؤلفون: | Yamaguchi D; Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, Kyoto, Japan.; Laboratory of Pharmacological and Experimental Therapeutics, Kyoto Pharmaceutical University, Kyoto, Japan., Kamoshida G; Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, Kyoto, Japan.; Department of Infection Control Science, Meiji Pharmaceutical University, Tokyo, Japan., Kawakubo S; Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, Kyoto, Japan., Azuma S; Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, Kyoto, Japan., Tsuji T; Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, Kyoto, Japan., Kitada N; Graduate School of Informatics and Engineering, The University of Electro-Communications, Chofu, Japan., Saito-Moriya R; Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Tokyo, Japan., Yamada N; Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, Kyoto, Japan., Tanaka R; Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, Kyoto, Japan., Okuda A; Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, Kyoto, Japan., Ueyama K; Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, Kyoto, Japan., Isaka S; Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, Kyoto, Japan., Tomita M; Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, Kyoto, Japan., Nakano R; Department of Microbiology and Infectious Diseases, Nara Medical University, Nara, Japan., Morita Y; Department of Infection Control Science, Meiji Pharmaceutical University, Tokyo, Japan., Yano H; Department of Microbiology and Infectious Diseases, Nara Medical University, Nara, Japan., Maki SA; Graduate School of Informatics and Engineering, The University of Electro-Communications, Chofu, Japan., Yahiro K; Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, Kyoto, Japan., Kato S; Laboratory of Pharmacological and Experimental Therapeutics, Kyoto Pharmaceutical University, Kyoto, Japan. |
| المصدر: | Microbiology spectrum [Microbiol Spectr] 2024 Sep 17, pp. e0082824. Date of Electronic Publication: 2024 Sep 17. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : ASM Press, 2013- |
| مستخلص: | In vivo imaging of bacterial infection models enables noninvasive and temporal analysis of individuals, enhancing our understanding of infectious disease pathogenesis. Conventional in vivo imaging methods for bacterial infection models involve the insertion of the bacterial luciferase LuxCDABE into the bacterial genome, followed by imaging using an expensive ultrasensitive charge-coupled device (CCD) camera. However, issues such as limited light penetration into the body and lack of versatility have been encountered. We focused on near-infrared (NIR) light, which penetrates the body effectively, and attempted to establish an in vivo imaging method to evaluate the number of lung-colonizing bacteria during the course of bacterial pneumonia. This was achieved by employing a novel versatile system that combines plasmid-expressing firefly luciferase bacteria, NIR substrate, and an inexpensive, scientific complementary metal-oxide semiconductor (sCMOS) camera. The D-luciferin derivative "TokeOni," capable of emitting NIR bioluminescence, was utilized in a mouse lung infection model of Acinetobacter baumannii , an opportunistic pathogen that causes pneumonia and is a concern due to drug resistance. TokeOni exhibited the highest sensitivity in detecting bacteria colonizing the mouse lungs compared with other detection systems such as LuxCDABE, enabling the monitoring of changes in bacterial numbers over time and the assessment of antimicrobial agent efficacy. Additionally, it was effective in detecting A. baumannii clinical isolates and Klebsiella pneumoniae . The results of this study are expected to be used in the analysis of animal models of infectious diseases for assessing the efficacy of therapeutic agents and understanding disease pathogenesis. Importance: Conventional animal models of infectious diseases have traditionally relied upon average assessments involving numerous individuals, meaning they do not directly reflect changes in the pathology of an individual. Moreover, in recent years, ethical concerns have resulted in the demand to reduce the number of animals used in such models. Although in vivo imaging offers an effective approach for longitudinally evaluating the pathogenesis of infectious diseases in individual animals, a standardized method has not yet been established. To our knowledge, this study is the first to develop a highly versatile in vivo pulmonary bacterial quantification system utilizing near-infrared luminescence, plasmid-mediated expression of firefly luciferase in bacteria, and a scientific complementary metal-oxide semiconductor camera. Our research holds promise as a useful tool for assessing the efficacy of therapeutic drugs and pathogenesis of infectious diseases. |
| فهرسة مساهمة: | Keywords: Acinetobacter baumannii; In vivo imaging; TokeOni; antibacterial therapy; bacterial pneumonia; near-infrared bioluminescence; scientific complementary metal-oxide semiconductor (sCMOS) camera |
| تواريخ الأحداث: | Date Created: 20240917 Latest Revision: 20240917 |
| رمز التحديث: | 20240918 |
| DOI: | 10.1128/spectrum.00828-24 |
| PMID: | 39287455 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2165-0497 |
|---|---|
| DOI: | 10.1128/spectrum.00828-24 |