دورية أكاديمية
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Non-thermal atmospheric pressure plasma induces selective cancer cell apoptosis by modulating redox homeostasis.

التفاصيل البيبلوغرافية
العنوان: Non-thermal atmospheric pressure plasma induces selective cancer cell apoptosis by modulating redox homeostasis.
المؤلفون: Yun JH; Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Ewha Womans University, Seoul, Korea, 07985., Yang YH; Department of Biomedical Sciences, Graduate School of Medicine, Ajou University, Suwon, Korea, 16499., Han CH; Department of Otolaryngology, School of Medicine, Ajou University, Suwon, Korea, 16499., Kang SU; Department of Otolaryngology, School of Medicine, Ajou University, Suwon, Korea, 16499. cows79@ajou.ac.kr., Kim CH; Department of Otolaryngology, School of Medicine, Ajou University, Suwon, Korea, 16499. ostium@ajou.ac.kr.
المصدر: Cell communication and signaling : CCS [Cell Commun Signal] 2024 Sep 26; Vol. 22 (1), pp. 452. Date of Electronic Publication: 2024 Sep 26.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101170464 Publication Model: Electronic Cited Medium: Internet ISSN: 1478-811X (Electronic) Linking ISSN: 1478811X NLM ISO Abbreviation: Cell Commun Signal Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : BioMed Central, c2003-
مواضيع طبية MeSH: Plasma Gases*/pharmacology , Apoptosis*/drug effects , Reactive Oxygen Species*/metabolism , Oxidation-Reduction*/drug effects , NF-E2-Related Factor 2*/metabolism , NF-E2-Related Factor 2*/genetics , Homeostasis*/drug effects, Humans ; Cell Line, Tumor ; Mitochondria/metabolism ; Mitochondria/drug effects ; Autophagy/drug effects
مستخلص: Background: Anticancer treatments aim to selectively target cancer cells without harming normal cells. While non-thermal atmospheric pressure plasma (NTAPP) has shown anticancer potential across various studies, the mechanisms behind its selective action on cancer cells remain inadequately understood. This study explores the mechanism of NTAPP-induced selective cell death and assesses its application in cancer therapy.
Methods: We treated HT1080 fibrosarcoma cells with NTAPP and assessed the intracellular levels of mitochondria-derived reactive oxygen species (ROS), mitochondrial function, and cell death mechanisms. We employed N-acetylcysteine to investigate ROS's role in NTAPP-induced cell death. Additionally, single-cell RNA sequencing was used to compare gene expression in NTAPP-treated HT1080 cells and human normal fibroblasts (NF). Western blotting and immunofluorescence staining examined the expression and nuclear translocation of nuclear factor erythroid 2-related factor 2 (NRF2), a key antioxidant gene transcription factor. We also evaluated autophagy activity through fluorescence staining and transmission electron microscopy.
Results: NTAPP treatment increased ROS levels and induced mitochondrial dysfunction, leading to apoptosis in HT1080 cells. The involvement of ROS in selective cancer cell death was confirmed by N-acetylcysteine treatment. Distinct gene expression patterns were observed between NTAPP-treated NF and HT1080 cells, with NF showing upregulated antioxidant gene expression. Notably, NRF2 expression and nuclear translocation increased in NF but not in HT1080 cells. Furthermore, autophagy activity was significantly higher in normal cells compared to cancer cells.
Conclusions: Our study demonstrates that NTAPP induces selective cell death in fibrosarcoma cells through the downregulation of the NRF2-induced ROS scavenger system and inhibition of autophagy. These findings suggest NTAPP's potential as a cancer therapy that minimizes damage to normal cells while effectively targeting cancer cells.
(© 2024. The Author(s).)
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معلومات مُعتمدة: RS-2023-00273483 Ministry of Science, ICT and Future Planning; HR21C1003 Ministry of Health and Welfare; 2021003350001 Ministry of Environment; RS-2024-00438448 Republic of Korea Korea Health Industry Development Institute
فهرسة مساهمة: Keywords: Autophagy; Fibrosarcoma; NRF2; Non-thermal atmospheric pressure plasma; Reactive oxygen species
المشرفين على المادة: 0 (Plasma Gases)
0 (Reactive Oxygen Species)
0 (NF-E2-Related Factor 2)
0 (NFE2L2 protein, human)
تواريخ الأحداث: Date Created: 20240926 Date Completed: 20240927 Latest Revision: 20240926
رمز التحديث: 20240927
DOI: 10.1186/s12964-024-01810-8
PMID: 39327567
قاعدة البيانات: MEDLINE
الوصف
تدمد:1478-811X
DOI:10.1186/s12964-024-01810-8