التفاصيل البيبلوغرافية
| العنوان: |
Posterior capsule opacification. An in vitro model. |
| المؤلفون: |
McDonnell PJ, Rowen SL, Glaser BM, Sato M |
| المصدر: |
Archives of ophthalmology (Chicago, Ill. : 1960) [Arch Ophthalmol] 1985 Sep; Vol. 103 (9), pp. 1378-81. |
| نوع المنشور: |
Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: American Medical Association Country of Publication: United States NLM ID: 7706534 Publication Model: Print Cited Medium: Print ISSN: 0003-9950 (Print) Linking ISSN: 00039950 NLM ISO Abbreviation: Arch Ophthalmol Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Chicago, IL : American Medical Association |
| مواضيع طبية MeSH: |
Cataract/*pathology, Animals ; Cataract/etiology ; Cataract/prevention & control ; Cattle ; Cells, Cultured ; Epithelium/ultrastructure ; Lens Capsule, Crystalline/ultrastructure ; Microscopy, Electron ; Mitochondria/ultrastructure ; Rabbits |
| مستخلص: |
Posterior capsule opacification results from migration of lens epithelial cells onto the posterior capsule, where they produce collagen and contract to cause wrinkling of the capsule. We have designed an in vitro model that reproduces the process of opacification. Lens capsules removed from enucleated bovine eyes are placed within specially designed holders, creating a culture dish with the capsule as its bottom surface. Albino rabbit lens epithelial cells are then plated on the capsule. These capsules develop clinical and ultrastructural features of opacification very similar to those observed in humans. Membranes composed of confluent epithelial cells closely resemble the cellular membranes observed with specular microscopy in human patients. Transmission electron microscopy demonstrates that a membrane several cells thick forms on the capsules. This model allows careful morphologic, metabolic, and biochemical studies of the process of opacification and the investigation of therapies designed to prevent opacification of the posterior capsule. |
| تواريخ الأحداث: |
Date Created: 19850901 Date Completed: 19851003 Latest Revision: 20190704 |
| رمز التحديث: |
20221208 |
| DOI: |
10.1001/archopht.1985.01050090130047 |
| PMID: |
4038131 |
| قاعدة البيانات: |
MEDLINE |
