دورية أكاديمية
Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies.
| العنوان: | Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies. |
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| المؤلفون: | Ross AH, Grob P, Bothwell M, Elder DE, Ernst CS, Marano N, Ghrist BF, Slemp CC, Herlyn M, Atkinson B, et. al. |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1984 Nov; Vol. 81 (21), pp. 6681-5. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print Cited Medium: Print ISSN: 0027-8424 (Print) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
| مواضيع طبية MeSH: | Antibodies, Monoclonal*, Melanoma/*metabolism , Receptors, Cell Surface/*metabolism, Affinity Labels ; Animals ; Antigens, Neoplasm/immunology ; Cell Line ; Cross-Linking Reagents ; Histocytochemistry ; Humans ; Immunoenzyme Techniques ; Immunosorbent Techniques ; Mice ; Mice, Inbred BALB C ; Receptors, Cell Surface/immunology ; Receptors, Nerve Growth Factor |
| مستخلص: | The nerve growth factor (NGF) receptor was characterized by using a new series of anti-receptor monoclonal antibodies (MAbs). These MAbs (i) showed significantly greater reactivity with a melanoma cell line expressing higher levels of NGF receptor, (ii) inhibited the binding of 125I-labeled NGF to its receptor, and (iii) immunoprecipitated both metabolically labeled and 125I-labeled NGF affinity-labeled receptor. These experiments defined the receptor as a 75-kDa cell-surface protein. The NGF receptor was visualized by immunoperoxidase staining in tissue sections of human nevi, melanomas, neurofibromas, a pheochromocytoma, and peripheral nerves. Uniform staining of the cytoplasm suggests that, in addition to cell-surface NGF receptors, there is a population of intracellular receptors. |
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| معلومات مُعتمدة: | CA-21124 United States CA NCI NIH HHS; CA-25874 United States CA NCI NIH HHS; CA-32952 United States CA NCI NIH HHS |
| المشرفين على المادة: | 0 (Affinity Labels) 0 (Antibodies, Monoclonal) 0 (Antigens, Neoplasm) 0 (Cross-Linking Reagents) 0 (Receptors, Cell Surface) 0 (Receptors, Nerve Growth Factor) |
| تواريخ الأحداث: | Date Created: 19841101 Date Completed: 19841219 Latest Revision: 20220309 |
| رمز التحديث: | 20240627 |
| مُعرف محوري في PubMed: | PMC391994 |
| DOI: | 10.1073/pnas.81.21.6681 |
| PMID: | 6093111 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0027-8424 |
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| DOI: | 10.1073/pnas.81.21.6681 |