دورية أكاديمية
Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies.
العنوان: | Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies. |
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المؤلفون: | Ross AH, Grob P, Bothwell M, Elder DE, Ernst CS, Marano N, Ghrist BF, Slemp CC, Herlyn M, Atkinson B, et. al. |
المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1984 Nov; Vol. 81 (21), pp. 6681-5. |
نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
اللغة: | English |
بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print Cited Medium: Print ISSN: 0027-8424 (Print) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
مواضيع طبية MeSH: | Antibodies, Monoclonal*, Melanoma/*metabolism , Receptors, Cell Surface/*metabolism, Affinity Labels ; Animals ; Antigens, Neoplasm/immunology ; Cell Line ; Cross-Linking Reagents ; Histocytochemistry ; Humans ; Immunoenzyme Techniques ; Immunosorbent Techniques ; Mice ; Mice, Inbred BALB C ; Receptors, Cell Surface/immunology ; Receptors, Nerve Growth Factor |
مستخلص: | The nerve growth factor (NGF) receptor was characterized by using a new series of anti-receptor monoclonal antibodies (MAbs). These MAbs (i) showed significantly greater reactivity with a melanoma cell line expressing higher levels of NGF receptor, (ii) inhibited the binding of 125I-labeled NGF to its receptor, and (iii) immunoprecipitated both metabolically labeled and 125I-labeled NGF affinity-labeled receptor. These experiments defined the receptor as a 75-kDa cell-surface protein. The NGF receptor was visualized by immunoperoxidase staining in tissue sections of human nevi, melanomas, neurofibromas, a pheochromocytoma, and peripheral nerves. Uniform staining of the cytoplasm suggests that, in addition to cell-surface NGF receptors, there is a population of intracellular receptors. |
References: | Physiol Rev. 1968 Jul;48(3):534-69. (PMID: 4875350) Proc Natl Acad Sci U S A. 1972 Dec;69(12):3556-60. (PMID: 4509314) Proc Natl Acad Sci U S A. 1977 Feb;74(2):565-9. (PMID: 265522) J Biol Chem. 1979 Jul 10;254(13):5972-82. (PMID: 571871) Proc Natl Acad Sci U S A. 1980 Jul;77(7):4188-92. (PMID: 6254014) Proc Natl Acad Sci U S A. 1980 Aug;77(8):4751-5. (PMID: 6933521) Cancer Res. 1980 Oct;40(10):3602-9. (PMID: 6159966) Cancer Res. 1981 Jul;41(7):2723-7. (PMID: 7248940) Cell. 1981 Jun;24(3):867-74. (PMID: 7195778) J Biol Chem. 1981 Sep 25;256(18):9419-24. (PMID: 6270084) J Cell Biol. 1982 Sep;94(3):710-7. (PMID: 6290502) Biochim Biophys Acta. 1982 Dec 8;693(1):205-12. (PMID: 6295482) J Biol Chem. 1983 Mar 10;258(5):3370-5. (PMID: 6298234) J Biol Chem. 1983 Mar 25;258(6):3741-9. (PMID: 6300053) J Neurochem. 1983 Jun;40(6):1654-63. (PMID: 6304252) EMBO J. 1983;2(6):879-85. (PMID: 6315419) J Biol Chem. 1983 Dec 10;258(23):14136-43. (PMID: 6315720) Proc Natl Acad Sci U S A. 1983 Nov;80(22):6819-23. (PMID: 6316327) Cancer Invest. 1983;1(3):215-24. (PMID: 6667406) Nature. 1984 Mar 1-7;308(5954):69-72. (PMID: 6700714) J Biol Chem. 1984 Jun 10;259(11):6882-9. (PMID: 6327698) Hybridoma. 1982;1(4):413-21. (PMID: 6765324) |
معلومات مُعتمدة: | CA-21124 United States CA NCI NIH HHS; CA-25874 United States CA NCI NIH HHS; CA-32952 United States CA NCI NIH HHS |
المشرفين على المادة: | 0 (Affinity Labels) 0 (Antibodies, Monoclonal) 0 (Antigens, Neoplasm) 0 (Cross-Linking Reagents) 0 (Receptors, Cell Surface) 0 (Receptors, Nerve Growth Factor) |
تواريخ الأحداث: | Date Created: 19841101 Date Completed: 19841219 Latest Revision: 20220309 |
رمز التحديث: | 20240627 |
مُعرف محوري في PubMed: | PMC391994 |
DOI: | 10.1073/pnas.81.21.6681 |
PMID: | 6093111 |
قاعدة البيانات: | MEDLINE |
تدمد: | 0027-8424 |
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DOI: | 10.1073/pnas.81.21.6681 |