دورية أكاديمية
[Interaction of SV40 T-antigen with homologous and heterologous DNA].
| العنوان: | [Interaction of SV40 T-antigen with homologous and heterologous DNA]. |
|---|---|
| عنوان ترانسليتريتد: | Vzaimodeĭstvie T-antigena virusa SV-40 s gomologichnoĭ i geterologichnymi DNK. |
| المؤلفون: | Polotskaia AV, Drize OB, Shliankevich MA, Shapot VS |
| المصدر: | Biokhimiia (Moscow, Russia) [Biokhimiia] 1981 Jun; Vol. 46 (6), pp. 1102-8. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Izdatelstvo Nauka Country of Publication: Russia (Federation) NLM ID: 0372667 Publication Model: Print Cited Medium: Print ISSN: 0320-9725 (Print) Linking ISSN: 03209725 NLM ISO Abbreviation: Biokhimiia Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Moskva : Izdatelstvo Nauka Original Publication: [Moskva] : Obʺedinennoe nauchno-tekhnicheskoe izd-vo, |
| مواضيع طبية MeSH: | Cell Transformation, Viral*, Antigens, Neoplasm/*immunology , Antigens, Viral/*immunology , DNA/*immunology , DNA, Viral/*immunology , Simian virus 40/*immunology, Antigen-Antibody Complex ; Antigens, Viral, Tumor ; Kinetics |
| مستخلص: | Using the DNA filter binding assay, the effects of ionic strength and pH on SV40 T-antigen interaction with viral DNA were studied. The apparent association constants for T-antigen binding to SV40 DNA in Scatchard coordinates in the presence of 40 mM NaCl are equal to 0.67 . 10(6) M-1 (pH 6.0) and 0.86 x 10(7) M-1 (pH 7.4). These data indicate that the interaction between T-antigen and SV40 DNA is more specific at pH 7.4. The coincident values of association constants for T-antigen binding to viral and cellular DNAs (Ka = 0.9 x 10(7) M-1 for cellular DNA) at pH 7.4 and the absence of competition between the two DNA species upon binding with T-antigen suggest that viral and cellular DNAs possess similar sites for T-antigen binding. Denatured DNA competes with viral DNA only at pH 6.0, when the T-antigen--SV40 DNA interaction is less specific. |
| المشرفين على المادة: | 0 (Antigen-Antibody Complex) 0 (Antigens, Neoplasm) 0 (Antigens, Viral) 0 (Antigens, Viral, Tumor) 0 (DNA, Viral) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 19810601 Date Completed: 19811029 Latest Revision: 20070723 |
| رمز التحديث: | 20231215 |
| PMID: | 6266518 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0320-9725 |
|---|