دورية أكاديمية
A simple method for the simultaneous measurement of sodium and chloride fluxes across transporting epithelia.
| العنوان: | A simple method for the simultaneous measurement of sodium and chloride fluxes across transporting epithelia. |
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| المؤلفون: | Thompson IG |
| المصدر: | Pflugers Archiv : European journal of physiology [Pflugers Arch] 1983 Mar 01; Vol. 396 (3), pp. 263-4. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 0154720 Publication Model: Print Cited Medium: Print ISSN: 0031-6768 (Print) Linking ISSN: 00316768 NLM ISO Abbreviation: Pflugers Arch Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin, New York, Springer. |
| مواضيع طبية MeSH: | Water-Electrolyte Balance*, Chlorides/*metabolism , Ion Channels/*metabolism , Sodium/*metabolism, Animals ; Biological Transport, Active ; Chlorine ; Epithelium/metabolism ; Humans ; Radioisotopes ; Sodium Radioisotopes |
| مستخلص: | A simple technique for the separation of 22Na and 36Cl radioactivity in the same solution is described. Two samples were taken from a solution containing both 22Na and 36Cl. One sample was precipitated with AgNO3 and the other was untreated. Samples were assayed for radioactivity by liquid scintillation spectrophotometry. Precipitation with AgNO3 removed 100% of the 36Cl activity from solution while having no effect on the 22Na radioactivity. Thus precipitated samples contained only 22Na radioactivity. Subtraction of the radioactivity in the supernatants of precipitated samples (i.e. 22Na) from the total radioactivity of untreated samples (i.e. 22Na and 36Cl) yielded the level of 36Cl activity in these samples. |
| References: | J Physiol. 1974 Oct;242(2):57P-58P. (PMID: 4455837) Am J Physiol. 1971 May;220(5):1388-96. (PMID: 5574657) |
| معلومات مُعتمدة: | AM-27064 United States AM NIADDK NIH HHS |
| المشرفين على المادة: | 0 (Chlorides) 0 (Ion Channels) 0 (Radioisotopes) 0 (Sodium Radioisotopes) 4R7X1O2820 (Chlorine) 9NEZ333N27 (Sodium) |
| تواريخ الأحداث: | Date Created: 19830301 Date Completed: 19830610 Latest Revision: 20190726 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1007/BF00587865 |
| PMID: | 6302636 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 0031-6768 |
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| DOI: | 10.1007/BF00587865 |