دورية أكاديمية
A simplified micro ELISA procedure for the measurement of platelet-associated IgG (PAIgG).
| العنوان: | A simplified micro ELISA procedure for the measurement of platelet-associated IgG (PAIgG). |
|---|---|
| المؤلفون: | Yesus YW, Scrivner DL, Mitchell MA, Mitra R |
| المصدر: | American journal of clinical pathology [Am J Clin Pathol] 1984 Jan; Vol. 81 (1), pp. 81-4. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0370470 Publication Model: Print Cited Medium: Print ISSN: 0002-9173 (Print) Linking ISSN: 00029173 NLM ISO Abbreviation: Am J Clin Pathol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2016- : Oxford : Oxford University Press Original Publication: Philadelphia [etc.] Lippincott [etc.] |
| مواضيع طبية MeSH: | Immunoenzyme Techniques*, Blood Platelets/*immunology , Enzyme-Linked Immunosorbent Assay/*methods , Immunoglobulin G/*analysis, Autoantibodies/analysis ; Humans ; Platelet Count ; Purpura, Thrombocytopenic/immunology ; Thrombocytopenia/immunology |
| مستخلص: | A simplified micro ELISA procedure to measure platelet-associated IgG is described. The platelet-bound IgG first is extracted into the fluid phase by solubilizing washed platelets in 0.1% triton X-100. The solubilized IgG in the extract and IgG standards are incubated in microtiter wells previously coated with antihuman IgG. The IgG in the standards and extract bind to the solid phase antihuman IgG. The bound IgG then is measured by the addition of peroxidase labeled antihuman IgG and appropriate substrate. With this method platelets from normal controls were found to have 1.7 +/- 0.6 fg IgG/platelet (mean +/- SD). Platelets from patients with ATP had values that were two to seven times the control values. The relative advantage of this technic is discussed. |
| المشرفين على المادة: | 0 (Autoantibodies) 0 (Immunoglobulin G) |
| تواريخ الأحداث: | Date Created: 19840101 Date Completed: 19840215 Latest Revision: 20190511 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1093/ajcp/81.1.81 |
| PMID: | 6362394 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0002-9173 |
|---|---|
| DOI: | 10.1093/ajcp/81.1.81 |