دورية أكاديمية
Rapid screening assay for toxic shock syndrome toxin production by Staphylococcus aureus.
| العنوان: | Rapid screening assay for toxic shock syndrome toxin production by Staphylococcus aureus. |
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| المؤلفون: | Weckbach LS, Thompson MR, Staneck JL, Bonventre PF |
| المصدر: | Journal of clinical microbiology [J Clin Microbiol] 1984 Jul; Vol. 20 (1), pp. 18-22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7505564 Publication Model: Print Cited Medium: Print ISSN: 0095-1137 (Print) Linking ISSN: 00951137 NLM ISO Abbreviation: J Clin Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Bacterial Toxins* , Superantigens*, Enterotoxins/*biosynthesis , Shock, Septic/*microbiology , Staphylococcus aureus/*metabolism, Bacteriological Techniques ; Female ; Humans ; Immunodiffusion |
| مستخلص: | A rapid immunoblot assay (TST-blot) was developed and used to screen Staphylococcus aureus isolates for toxic shock syndrome toxin (TST) production. Growth from an 18-h stab inoculum of S. aureus on brain heart infusion agar was transferred directly to a nitrocellulose sheet. Nonspecific protein binding sites were blocked with bovine serum albumin, and the nitrocellulose sheet was incubated with affinity-purified antibody to TST, followed by incubation with horseradish peroxidase-conjugated protein A. Toxin was visualized by detection of the peroxidase-conjugated protein A-anti TST-TST complex with 4-chloro-1-napthol. The sensitivities and specificities of the TST-blot and Ouchterlony microslide immunodiffusion assay were compared by screening 141 S. aureus isolates for TST production. In both assays, 53 of 141 isolates produced detectable levels of TST, whereas 88 isolates produced no toxin. A 100% concordance was found between the two assays. The TST-blot yielded the same results in less than 24 h as those yielded by the 3-day immunodiffusion assay. Thus, this rapid method for detection of TST in multiple samples appears to be well suited for diagnostic and epidemiological studies. Furthermore, it would appear to be ideal for use in TST genetics research. |
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| المشرفين على المادة: | 0 (Bacterial Toxins) 0 (Enterotoxins) 0 (Superantigens) 0 (enterotoxin F, Staphylococcal) |
| تواريخ الأحداث: | Date Created: 19840701 Date Completed: 19840827 Latest Revision: 20210526 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC271237 |
| DOI: | 10.1128/jcm.20.1.18-22.1984 |
| PMID: | 6430953 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0095-1137 |
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| DOI: | 10.1128/jcm.20.1.18-22.1984 |