دورية أكاديمية
Affinity separation and partial characterization of serologically active Leishmania donovani antigens.
| العنوان: | Affinity separation and partial characterization of serologically active Leishmania donovani antigens. |
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| المؤلفون: | Rassam MB, Al-Nuaimi MS, Issa FS |
| المصدر: | Annals of tropical medicine and parasitology [Ann Trop Med Parasitol] 1984 Feb; Vol. 78 (1), pp. 35-41. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 2985178R Publication Model: Print Cited Medium: Print ISSN: 0003-4983 (Print) Linking ISSN: 00034983 NLM ISO Abbreviation: Ann Trop Med Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2016- : Abingdon : Taylor & Francis Original Publication: London : Academic Press |
| مواضيع طبية MeSH: | Antigens/*analysis , Leishmania/*immunology , Leishmaniasis, Visceral/*immunology, Animals ; Antibody Formation ; Antigens/immunology ; Chromatography, Affinity ; Immunization ; Immunoelectrophoresis ; Isoelectric Point ; Molecular Weight ; Rabbits |
| مستخلص: | Leishmania donovani-soluble antigens capable of antibody production in rabbits were separated from the total antigenic make-up of the parasite by adsorption on to anti-Leishmania immunoglobulins coupled to CNBr-activated Sepharose 4B (ALIG). Immune rabbit sera were produced either by intravenous inoculation of living promastigotes or by intradermal and subcutaneous inoculation of soluble antigens. Approximately four times as much soluble antigen was bound to ALIG produced by i.v. than i.d. and s.c. inoculation although the percentage recovery was less. The antigenic fractions recovered were subjected to immunoelectrophoresis, isoelectric point and molecular weight determination. The results of affinity chromatography indicate high antigen-antibody interaction affinity, the recovery of at least 28 antigens (with 0.15 M NaCl, pH 11.0) and complete loss of serological activity (determined by immunoelectrophoresis) on treatment with 8 M urea. |
| المشرفين على المادة: | 0 (Antigens) |
| تواريخ الأحداث: | Date Created: 19840201 Date Completed: 19840620 Latest Revision: 20190516 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1080/00034983.1984.11811770 |
| PMID: | 6721613 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 0003-4983 |
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| DOI: | 10.1080/00034983.1984.11811770 |