دورية أكاديمية
Culture-produced subendothelium. I. Platelet interaction and properties.
| العنوان: | Culture-produced subendothelium. I. Platelet interaction and properties. |
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| المؤلفون: | Booyse FM, Quarfoot AJ, Feder S |
| المصدر: | Haemostasis [Haemostasis] 1982; Vol. 11 (1), pp. 49-61. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Karger Country of Publication: Switzerland NLM ID: 0371574 Publication Model: Print Cited Medium: Print ISSN: 0301-0147 (Print) Linking ISSN: 03010147 NLM ISO Abbreviation: Haemostasis Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, New York, Karger. |
| مواضيع طبية MeSH: | Aorta* , Platelet Adhesiveness*, Blood Platelets/*physiology , Cytoskeleton/*physiology , Endothelium/*ultrastructure, Animals ; Cell Communication ; Cells, Cultured ; Culture Media ; Factor VIII/physiology ; Hydrogen-Ion Concentration ; Microscopy, Electron ; Protease Inhibitors/physiology ; Swine ; von Willebrand Factor/physiology |
| مستخلص: | Culture-produced subendothelium (SE) has been prepared from cultured porcine aortic endothelial cells (ECs) by a rapid freeze-thaw, ice-shearing method. En face preparations of this in situ SE material are essentially free of intact or damaged cells and cell debris and consisted of an extensive meshwork of microfibrillar and amorphous material. Washed porcine platelets reacted extensively with this SE material and were associated with the SE as single adherent platelets, single spread platelets, and varying-sized platelet aggregates or 'microthrombi'. Platelet aggregates were associated only with the damaged or frayed edges of the SE, and the platelets had undergone extensive SE-induced contraction and degranulation, as indicated by transmission electron microscopy. Platelet-SE interaction was affected by pH, calcium, platelet concentration, rapid shaking and exposure time. Platelet-SE interaction was significantly enhanced by the addition of 0.1-1% citrated plasma or purified porcine F.VIIIR:WF. Pretreatment of the SE with thrombin, elastase, neuraminidase or hyaluronidase had no effect on platelet-SE interaction, whereas pretreatment with pepsin, plasmin, trypsin, alpha-chymotrypsin or collagenase decreased or completely abolished all platelet-SE interaction. Extraction of the SE with various solutions (high salt, detergents, etc.) had no effect on platelet-SE interaction, only solutions containing sodium dodecyl sulfate completely abolished all platelet-SE interaction. |
| معلومات مُعتمدة: | HL-17430 United States HL NHLBI NIH HHS; HL-17973 United States HL NHLBI NIH HHS |
| المشرفين على المادة: | 0 (Culture Media) 0 (Protease Inhibitors) 0 (von Willebrand Factor) 9001-27-8 (Factor VIII) |
| تواريخ الأحداث: | Date Created: 19820101 Date Completed: 19820614 Latest Revision: 20180216 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1159/000214640 |
| PMID: | 6802725 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0301-0147 |
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| DOI: | 10.1159/000214640 |