دورية أكاديمية
Effects of extracellular calcium removal and anoxia on isolated rat myocytes.
| العنوان: | Effects of extracellular calcium removal and anoxia on isolated rat myocytes. |
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| المؤلفون: | Cheung JY, Thompson IG, Bonventre JV |
| المصدر: | The American journal of physiology [Am J Physiol] 1982 Sep; Vol. 243 (3), pp. C184-90. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 0370511 Publication Model: Print Cited Medium: Print ISSN: 0002-9513 (Print) Linking ISSN: 00029513 NLM ISO Abbreviation: Am J Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Bethesda, MD : American Physiological Society Original Publication: Washington [etc.] American Physiological Society. |
| مواضيع طبية MeSH: | Calcium/*metabolism , Hypoxia/*metabolism , Myocardium/*metabolism, Adenosine Triphosphate/metabolism ; Animals ; Male ; Microscopy, Electron ; Myocardium/cytology ; Perfusion ; Phosphocreatine/metabolism ; Potassium/metabolism ; Rats ; Rats, Inbred Strains ; Sodium/metabolism |
| مستخلص: | Calcium-tolerant myocytes were isolated from adult rat ventricles by successive perfusion and incubation with buffer containing collagenase and hyaluronidase. Greater than 70% of the cells excluded trypan blue, maintained normal morphology, and contracted in response to an externally applied electric field. We have characterized metabolic defects present in isolated calcium-tolerance myocytes when exposed to low concentrations of extracellular calcium under aerobic and anaerobic conditions. In control cells exposed to 1.25 mM Ca2+, the following metabolic parameters were measured (in mumol/g protein): adenosine triphosphate (ATP) 28.8 +/- 3.3, creatine phosphate (CrP) 49.1 +/- 7.5, intracellular Na+ 37.7 +/- 8.1, intracellular K+ 352.9 +/- 49.3, cellular Ca2+ 12.3 +/- 1.8, as well as rate of protein synthesis 0.34 +/- 0.03 mumol . g protein-1 . h-1. In aerobic cells incubated in medium without added Ca2+, the corresponding values (in mumol/g protein) were ATP 27.9 +/- 4.4, CrP 25.3 +/- 4.3, intracellular Na+ 130.9 +/- 23.1, intracellular K+ 217.2 +/- 32.0, cellular Ca2+ 3.9 +/- 1.0, and rate of protein synthesis 0.09 +/- 0.02 mumol . g protein-1 . h-1. These data indicated major metabolic aberrations in myocytes exposed to medium low in Ca2+ (less than 10 microM). Metabolic depression was most severe in cells incubated in the absence of both Ca2+ and O2. It is postulated that Ca2+ removal resulted in an increase in Na+ and K+ permeability, causing a net gain of intracellular Na+ and loss of intracellular K+. These ionic shifts might stimulate the activity of membrane-associated Na+-K+-ATPase, accounting for lower levels of CrP. |
| معلومات مُعتمدة: | AM-27957 United States AM NIADDK NIH HHS; HL-06378 United States HL NHLBI NIH HHS; HL-06664 United States HL NHLBI NIH HHS |
| المشرفين على المادة: | 020IUV4N33 (Phosphocreatine) 8L70Q75FXE (Adenosine Triphosphate) 9NEZ333N27 (Sodium) RWP5GA015D (Potassium) SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 19820901 Date Completed: 19821029 Latest Revision: 20171213 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1152/ajpcell.1982.243.3.C184 |
| PMID: | 7114249 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0002-9513 |
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| DOI: | 10.1152/ajpcell.1982.243.3.C184 |