دورية أكاديمية
Determination of midazolam and two metabolites of midazolam in human plasma by gas chromatography--negative chemical-ionization mass spectrometry.
| العنوان: | Determination of midazolam and two metabolites of midazolam in human plasma by gas chromatography--negative chemical-ionization mass spectrometry. |
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| المؤلفون: | Rubio F, Miwa BJ, Garland WA |
| المصدر: | Journal of chromatography [J Chromatogr] 1982 Dec 10; Vol. 233, pp. 157-65. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0427043 Publication Model: Print Cited Medium: Print NLM ISO Abbreviation: J Chromatogr Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier. |
| مواضيع طبية MeSH: | Benzodiazepines/*blood , Hypnotics and Sedatives/*blood, Adult ; Benzodiazepines/metabolism ; Gas Chromatography-Mass Spectrometry/methods ; Humans ; Hypnotics and Sedatives/metabolism ; Male ; Midazolam ; Preanesthetic Medication ; Time Factors ; Trimethylsilyl Compounds |
| مستخلص: | A method is described for measuring midazolam, a new anesthesia induction agent and hypnotic, and its hydroxymethyl and desmethyl metabolites in human plasma. Deuterated analogues of each compound are added to plasma as internal standards. The compounds are extracted from plasma with benzene containing 20% 1,2-dichloroethane and after removal of the extracting solvent are dissolved in a solution of bis-(trimethylsilyl)acetamide and acetonitrile. An aliquot of this solution is analyzed by gas chromatography--mass spectrometry with the mass spectrometer set to monitor in the gas chromatographic effluent the M.- ions of drug, metabolites and internal standards generated by methane electron-capture negative chemical ionization. For all three compounds, the limit of quantitation is 1 ng ml-1, and the precision (relative standard deviation) at a concentration of 5 ng ml-1 is less than 6%. Measurable amounts of the hydroxymethyl, but not the desmethyl, metabolite of midazolam could be found in the plasma of humans given either an intravenous or an oral dose of midazolam maleate. |
| المشرفين على المادة: | 0 (Hypnotics and Sedatives) 0 (Trimethylsilyl Compounds) 12794-10-4 (Benzodiazepines) R60L0SM5BC (Midazolam) |
| تواريخ الأحداث: | Date Created: 19821210 Date Completed: 19830415 Latest Revision: 20190629 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1016/s0378-4347(00)81742-9 |
| PMID: | 7161330 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.1016/s0378-4347(00)81742-9 |
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