التفاصيل البيبلوغرافية
| العنوان: |
On the leakage of acrosomal hyaluronidase from the spermatozoa of the buffalo (Bubalus bubalis). |
| المؤلفون: |
Ganguli NC, Kakar SS |
| المصدر: |
Reproduction, nutrition, developpement [Reprod Nutr Dev (1980)] 1980; Vol. 20 (3A), pp. 593-9. |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: Institut National De La Recherche Agronomique By Editions Scientifiques Elsevier Country of Publication: France NLM ID: 8005903 Publication Model: Print Cited Medium: Print ISSN: 0181-1916 (Print) Linking ISSN: 01811916 NLM ISO Abbreviation: Reprod Nutr Dev (1980) Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Paris : Institut National De La Recherche Agronomique By Editions Scientifiques Elsevier |
| مواضيع طبية MeSH: |
Acrosome/*enzymology , Buffaloes/*physiology , Hyaluronoglucosaminidase/*metabolism , Spermatozoa/*enzymology, Animals ; Freezing ; Male ; Semen ; Semen Preservation/methods ; Sonication ; Sperm Motility |
| مستخلص: |
The sonication method produced a quantitatively higher release of buffalo sperm hyaluronidase than the freeze-thaw technique. The released enzyme constituted 10 p. 100 of the total enzymatic activity of the fresh semen. Seminal plasma hyaluronidase activity was not correlated with the motility score of the semen sample. Unlike cattle semen, the seminal plasma enzyme level in buffalo semen stored at 37 degrees C showed a sharp rise, whereas samples stored at 0 degrees C evidenced negligible enzyme leakage. Dilution of semen in citric acid whey (CAW) at 5 or 37 degrees C significantly prevented the enzyme from leaking into the plasma, although more enzyme was released in extended semen when it was exposed to cold treatments. The enzyme was quite stable in both the seminal plasma and the acrosomal preparations during storage when stored at 5 degrees C for prolonged periods. |
| المشرفين على المادة: |
EC 3.2.1.35 (Hyaluronoglucosaminidase) |
| تواريخ الأحداث: |
Date Created: 19800101 Date Completed: 19830311 Latest Revision: 20191031 |
| رمز التحديث: |
20240628 |
| DOI: |
10.1051/rnd:19800401 |
| PMID: |
7349430 |
| قاعدة البيانات: |
MEDLINE |
