دورية أكاديمية
Measurement of vascular permeability in spinal cord using Evans Blue spectrophotometry and correction for turbidity.
| العنوان: | Measurement of vascular permeability in spinal cord using Evans Blue spectrophotometry and correction for turbidity. |
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| المؤلفون: | Warnick RE; Department of Neurosurgery, University of Cincinnati College of Medicine, OH 45267, USA., Fike JR, Chan PH, Anderson DK, Ross GY, Gutin PH |
| المصدر: | Journal of neuroscience methods [J Neurosci Methods] 1995 May; Vol. 58 (1-2), pp. 167-71. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7905558 Publication Model: Print Cited Medium: Print ISSN: 0165-0270 (Print) Linking ISSN: 01650270 NLM ISO Abbreviation: J Neurosci Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press. |
| مواضيع طبية MeSH: | Capillary Permeability/*physiology , Spinal Cord/*metabolism, Animals ; Evans Blue ; Female ; Formamides ; Nephelometry and Turbidimetry ; Rats ; Rats, Inbred F344 ; Spectrometry, Fluorescence |
| مستخلص: | Vascular permeability can be visualized by Evans Blue (EB) extravasation and quantified by spectrophotometry after formamide extraction of the tissue. However, formamide extracts show significant turbidity, which may contribute to the total optical density at the wavelength of measurement (e.g., 620 lambda). We developed a simple method for estimating the component of the total optical density of a dyed specimen contributed by turbidity. Our method, which uses a determination of turbidity made at another point of the light spectrum (740 lambda), was more precise than two other EB quantification techniques. We therefore recommend it for individual correction of formamide extracts of spinal cord specimens. The application of this technique to the brain remains to be determined. |
| معلومات مُعتمدة: | CA 09291 United States CA NCI NIH HHS; CA 13525 United States CA NCI NIH HHS |
| المشرفين على المادة: | 0 (Formamides) 45PG892GO1 (Evans Blue) |
| تواريخ الأحداث: | Date Created: 19950501 Date Completed: 19951130 Latest Revision: 20190830 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/0165-0270(94)00172-d |
| PMID: | 7475223 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0165-0270 |
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| DOI: | 10.1016/0165-0270(94)00172-d |