دورية أكاديمية
Functional significance of interaction of H-FABP with sulfated and nonsulfated taurine-conjugated bile salts in rat liver.
| العنوان: | Functional significance of interaction of H-FABP with sulfated and nonsulfated taurine-conjugated bile salts in rat liver. |
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| المؤلفون: | Dietrich A; Institut für Organische Chemie und Biochemie, Universität Freiburg, Germany., Dieminger W, Fuchte K, Stoll GH, Schlitz E, Gerok W, Kurz G |
| المصدر: | Journal of lipid research [J Lipid Res] 1995 Aug; Vol. 36 (8), pp. 1745-55. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0376606 Publication Model: Print Cited Medium: Print ISSN: 0022-2275 (Print) Linking ISSN: 00222275 NLM ISO Abbreviation: J Lipid Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York] : Elsevier Original Publication: Memphis, Lipid Research, inc. |
| مواضيع طبية MeSH: | Neoplasm Proteins* , Nerve Tissue Proteins*, Bile Acids and Salts/*metabolism , Carrier Proteins/*metabolism , Liver/*metabolism , Myelin P2 Protein/*metabolism , Sulfuric Acid Esters/*metabolism , Taurine/*metabolism, Affinity Labels ; Animals ; Biological Transport ; Cytosol/chemistry ; Cytosol/metabolism ; Electrophoresis, Polyacrylamide Gel ; Fatty Acid-Binding Protein 7 ; Fatty Acid-Binding Proteins ; Isoelectric Focusing ; Male ; Precipitin Tests ; Rats ; Rats, Wistar ; Solubility ; Subcellular Fractions/chemistry ; Subcellular Fractions/metabolism ; Taurolithocholic Acid/analogs & derivatives ; Taurolithocholic Acid/metabolism |
| مستخلص: | To identify the cytosolic proteins of rat hepatocytes involved in transcellular transport of sulfated and taurine-conjugated bile salts in comparison with only taurine-conjugated bile salts, photoaffinity labeling studies were performed with [3H]-7,7-ASLCT (7,7-azi-3 alpha-sulfatolithocholyl[2'-3H(N)]taurine), [3H]-7,7-ACT ((7,7-azi-3 alpha,12 alpha-dihydroxy-5 beta-cholan-24-oyl)- 2'-[2'-3H(N)]aminoethanesulfonate), and [3H]-7,7-ALCT (7,7-azilithocholyl[2'-3H(N)]taurine) using isolated hepatocytes and intact liver tissue. Photoaffinity labeling of isolated hepatocytes with [3H]-7,7-ASLCT on the one hand and with [3H]-7,7-ACT and [3H]-7,7-ALCT on the other resulted in a different labeling pattern of cytosolic polypeptides, without a relevant incorporation of radioactivity into subunits of glutathione transferases. This suggests that glutathione transferases play no role in the transport of dianionic or of monoanionic bile salts. With [3H]-7,7-ACT and [3H]-7,7-ALCT a moderate incorporation of radioactivity was found in polypeptides with apparent M(r)s of 33,000, 38,000, and 54,000, whereas with [3H]-7,7-ASLCT, a polypeptide with an apparent M(r) of 14,000, identified as H-FABP, was markedly and almost exclusively labeled. Photoaffinity labeling of specimens of intact liver tissue resulted in a labeling pattern of cytosolic polypeptides comparable to that obtained from photolabeled isolated hepatocytes. All results suggest that transcellular transport of dianionic sulfated as well as taurine-conjugated bile salts and of monoanionic taurine-conjugated bile salts follows different pathways. In intracellular transport of taurine-conjugated bile salts, several cytosolic polypeptides may have a function, whereas, in transport of taurine-conjugated 3 alpha-sulfato bile salts, only H-FABP appears to be involved. |
| المشرفين على المادة: | 0 (Affinity Labels) 0 (Bile Acids and Salts) 0 (Carrier Proteins) 0 (Fabp7 protein, rat) 0 (Fatty Acid-Binding Protein 7) 0 (Fatty Acid-Binding Proteins) 0 (Myelin P2 Protein) 0 (Neoplasm Proteins) 0 (Nerve Tissue Proteins) 0 (Sulfuric Acid Esters) 15324-65-9 (taurolithocholic acid 3-sulfate) 1EQV5MLY3D (Taurine) 516-90-5 (Taurolithocholic Acid) |
| تواريخ الأحداث: | Date Created: 19950801 Date Completed: 19951222 Latest Revision: 20210217 |
| رمز التحديث: | 20221213 |
| PMID: | 7595095 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0022-2275 |
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