دورية أكاديمية
Changes in clusterin expression associated with light-induced retinal damage in rats.
| العنوان: | Changes in clusterin expression associated with light-induced retinal damage in rats. |
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| المؤلفون: | Wong P; National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA., Kutty RK, Darrow RM, Shivaram S, Kutty G, Fletcher RT, Wiggert B, Chader G, Organisciak DT |
| المصدر: | Biochemistry and cell biology = Biochimie et biologie cellulaire [Biochem Cell Biol] 1994 Nov-Dec; Vol. 72 (11-12), pp. 499-503. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Canadian Science Publishing Country of Publication: Canada NLM ID: 8606068 Publication Model: Print Cited Medium: Print ISSN: 0829-8211 (Print) Linking ISSN: 08298211 NLM ISO Abbreviation: Biochem Cell Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2011- : Ottawa, ON : Canadian Science Publishing Original Publication: Ottawa, Ont. : National Research Council of Canada, c1986- |
| مواضيع طبية MeSH: | Molecular Chaperones*, Glycoproteins/*biosynthesis , Light/*adverse effects , Retina/*metabolism , Retinal Degeneration/*metabolism, Animals ; Clusterin ; Male ; RNA, Messenger/analysis ; Rats ; Rats, Sprague-Dawley ; Retina/pathology ; Retina/radiation effects ; Retinal Degeneration/etiology ; Retinal Degeneration/pathology ; Thiourea/analogs & derivatives ; Thiourea/pharmacology |
| مستخلص: | Prolonged periods of high-intensity visible light exposure lead to photoreceptor cell degeneration, but the mechanism of damage is not understood. Increased clusterin mRNA levels have been found in several models of apoptosis, as well as in neurodegeneration. We report here that changes in clusterin mRNA levels are also associated with light-induced retinal damage in adult male albino rats. Animals previously maintained in darkness or a weak cyclic light environment were exposed to intense visible green light for up to 24 h. Some rats were pretreated with a synthetic antioxidant, dimethylthiourea (DMTU), which reduces photoreceptor cell degeneration. Clusterin mRNA steady-state levels increased with the duration of light exposure in both cyclic light and dark reared animals, suggesting that an apoptotic mechanism may be involved. Animals pretreated with DMTU showed a delay in the initial increase in clusterin mRNA levels, suggesting that oxidative damage is involved in the damage mechanism. However, the incomplete suppression of increasing steady-state clusterin mRNA levels by DMTU suggests that either oxidative damage triggers a second pathway or multiple damage mechanisms are induced in the retina by light exposure. |
| معلومات مُعتمدة: | EY01959 United States EY NEI NIH HHS |
| المشرفين على المادة: | 0 (Clusterin) 0 (Glycoproteins) 0 (Molecular Chaperones) 0 (RNA, Messenger) 8P30PMD17W (1,3-dimethylthiourea) GYV9AM2QAG (Thiourea) |
| تواريخ الأحداث: | Date Created: 19941101 Date Completed: 19951005 Latest Revision: 20190913 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1139/o94-067 |
| PMID: | 7654323 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0829-8211 |
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| DOI: | 10.1139/o94-067 |