دورية أكاديمية
Characterization of a CD34+ cell line established from a child with large cell cutaneous lymphoma.
| العنوان: | Characterization of a CD34+ cell line established from a child with large cell cutaneous lymphoma. |
|---|---|
| المؤلفون: | Lorenzana AN; Department of Pediatrics, University of Chicago., McCabe NR, Goodell WR, Zhang LQ, Miley D, Le Beau MM, Goldman S, Smith SD |
| المصدر: | Cancer [Cancer] 1993 Aug 01; Vol. 72 (3), pp. 931-7. |
| نوع المنشور: | Case Reports; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley Country of Publication: United States NLM ID: 0374236 Publication Model: Print Cited Medium: Print ISSN: 0008-543X (Print) Linking ISSN: 0008543X NLM ISO Abbreviation: Cancer Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2005- >: Hoboken, NJ : Wiley Original Publication: New York [etc.] Published for the American Cancer Society by J. Wiley [etc.] |
| مواضيع طبية MeSH: | Antigens, CD* , Antigens, Neoplasm*, Lymphoma, B-Cell/*immunology , Lymphoma, Large B-Cell, Diffuse/*immunology , Skin Neoplasms/*immunology , Tumor Cells, Cultured/*immunology, Antigens, CD34 ; Cell Division/drug effects ; Humans ; Immunoglobulin M/biosynthesis ; Immunophenotyping ; Infant ; Interleukin-6/pharmacology ; Lymphoma, B-Cell/genetics ; Lymphoma, B-Cell/pathology ; Lymphoma, Large B-Cell, Diffuse/genetics ; Lymphoma, Large B-Cell, Diffuse/pathology ; Male ; Receptors, Immunologic/analysis ; Receptors, Interleukin-6 ; Skin Neoplasms/etiology ; Skin Neoplasms/pathology |
| مستخلص: | Background: Lymphoma presenting with skin involvement has heterogeneous morphology and rarely is seen in children. To study the pathogenesis of this disease, lymphoma cells from a child with B-cell large cell lymphoma of the skin were cultured in vitro. Methods: Lymphoma cells cultured on a feeder layer under hypoxic conditions grew in vitro after a latency period of 2 weeks. Since interleukin-6 (IL-6) induces final differentiation of activated B-lymphocytes, the cell line was evaluated for the presence of IL-6 receptors and biologic response to IL-6. Results: An Epstein-Barr virus (EBV)-negative cell line (UoC-B2) was established which expressed CD34, CD45, HLA-DR, CD19, CD20, sIgM, sIgD, and lambda light chain. Good general concordance was observed between the patient's lymphoma and the cell line by comparing the immunophenotype, genotype, and karyotype. The UoC-B2 cells expressed surface IgM but did not secrete IgM into the culture media even in the presence of supplemental IL-6. Conclusions: A B-lymphoid cell line (UoC-B2) was established from a child with primary cutaneous lymphoma. The cells expressed cell surface IgM and receptors for IL-6 but supplemental IL-6 had no effect on IgM production or cell proliferation. |
| المشرفين على المادة: | 0 (Antigens, CD) 0 (Antigens, CD34) 0 (Antigens, Neoplasm) 0 (Immunoglobulin M) 0 (Interleukin-6) 0 (Receptors, Immunologic) 0 (Receptors, Interleukin-6) |
| تواريخ الأحداث: | Date Created: 19930801 Date Completed: 19930824 Latest Revision: 20190620 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1002/1097-0142(19930801)72:3<931::aid-cncr2820720344>3.0.co;2-# |
| PMID: | 7687517 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0008-543X |
|---|---|
| DOI: | 10.1002/1097-0142(19930801)72:3<931::aid-cncr2820720344>3.0.co;2-# |