دورية أكاديمية
Overexpression of Bcl-XS sensitizes MCF-7 cells to chemotherapy-induced apoptosis.
| العنوان: | Overexpression of Bcl-XS sensitizes MCF-7 cells to chemotherapy-induced apoptosis. |
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| المؤلفون: | Sumantran VN; Department of Internal Medicine, University of Michigan Comprehensive Cancer Center, Ann Arbor 48109-0724, USA., Ealovega MW, Nuñez G, Clarke MF, Wicha MS |
| المصدر: | Cancer research [Cancer Res] 1995 Jun 15; Vol. 55 (12), pp. 2507-10. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association for Cancer Research Country of Publication: United States NLM ID: 2984705R Publication Model: Print Cited Medium: Print ISSN: 0008-5472 (Print) Linking ISSN: 00085472 NLM ISO Abbreviation: Cancer Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Baltimore, Md. : American Association for Cancer Research Original Publication: Chicago [etc.] |
| مواضيع طبية MeSH: | Proto-Oncogene Proteins c-bcl-2* , Repressor Proteins*, Antineoplastic Agents/*toxicity , Apoptosis/*drug effects , Paclitaxel/*toxicity , Proto-Oncogene Proteins/*biosynthesis, Breast Neoplasms ; Cell Division/drug effects ; Cell Line ; Enzyme-Linked Immunosorbent Assay ; Humans ; Kanamycin Kinase ; Oncogene Proteins, Viral/metabolism ; Papillomaviridae/genetics ; Papillomaviridae/metabolism ; Phosphotransferases (Alcohol Group Acceptor)/biosynthesis ; Recombinant Proteins/biosynthesis ; Transfection ; Tumor Cells, Cultured ; bcl-X Protein |
| مستخلص: | Resistance to apoptosis plays an important role in tumors that are refractory to chemotherapy. We report that Bcl-XL, which functions like Bcl-2 to inhibit apoptosis, is highly expressed in MCF-7 human breast carcinoma cells. We used Bcl-XS, a dominant negative inhibitor of Bcl-2 and Bcl-XL, to demonstrate the role of these genes in modulating chemotherapy-induced apoptosis. Bcl-XS overexpressed in MCF-7 cells by stable transfection does not affect viability by itself but induces a marked increase in chemosensitivity to VP-16 or taxol. Using an ELISA assay which quantitates DNA damage, we demonstrate that this sensitization is due to apoptosis, suggesting the therapeutic utility of targeting this pathway. |
| معلومات مُعتمدة: | CA-61777 United States CA NCI NIH HHS; CA-64556 United States CA NCI NIH HHS; K04 CA64421-01 United States CA NCI NIH HHS |
| Gene Symbol: | bcl-x |
| المشرفين على المادة: | 0 (Antineoplastic Agents) 0 (BCL2L1 protein, human) 0 (E6 protein, Human papillomavirus type 16) 0 (Oncogene Proteins, Viral) 0 (Proto-Oncogene Proteins) 0 (Proto-Oncogene Proteins c-bcl-2) 0 (Recombinant Proteins) 0 (Repressor Proteins) 0 (bcl-X Protein) EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor)) EC 2.7.1.95 (Kanamycin Kinase) P88XT4IS4D (Paclitaxel) |
| تواريخ الأحداث: | Date Created: 19950615 Date Completed: 19950719 Latest Revision: 20151119 |
| رمز التحديث: | 20231215 |
| PMID: | 7780958 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0008-5472 |
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