دورية أكاديمية
Retrograde neuronal tracing with cholera toxin B subunit: comparison of three different visualization methods.
| العنوان: | Retrograde neuronal tracing with cholera toxin B subunit: comparison of three different visualization methods. |
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| المؤلفون: | Dederen PJ; Faculty of Medical Sciences, University of Nijmegen, Department of Anatomy and Embryology, The Netherlands., Gribnau AA, Curfs MH |
| المصدر: | The Histochemical journal [Histochem J] 1994 Nov; Vol. 26 (11), pp. 856-62. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 0163161 Publication Model: Print Cited Medium: Print ISSN: 0018-2214 (Print) Linking ISSN: 00182214 NLM ISO Abbreviation: Histochem J Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1999-c2002: Dordrecht : Kluwer Academic Publishers Original Publication: London, Chapman and Hall. |
| مواضيع طبية MeSH: | Cholera Toxin* , Fluorescent Dyes*, Immunohistochemistry/*methods , Motor Neurons/*cytology, Animals ; Cervix Uteri/innervation ; Female ; Fluorescein-5-isothiocyanate ; Forelimb ; Horseradish Peroxidase ; Rats ; Rats, Wistar ; Rhodamines |
| مستخلص: | In this report a comparison is made of three different visualization methods of rat cervical motoneurons retrogradely labelled with cholera toxin B subunit (CTb). CTb conjugates such as CTb-HRP and CTb-FITC or CTb-TRITC, which can be visualized after histochemical detection and by fluorescence microscopy, respectively. The following results were obtained. (1) Immunochemical detection of CTb with peroxidase and DAB-Ni incubation provides the best labelling of the cell bodies and their processes, whereas immunochemical detection with FITC produces less effective labelling of the dendrites. (2) Histochemical visualization of CTb-HRP conjugate gives results similar to those of CTb immunochemistry but produces a much more granular appearance of the label, which may affect the identification of distal dendrites. In addition, direct electron-microscopic analysis of labelled structures can be achieved. (3) CTb-FITC and CTb-TRITC visualization permit double-labelling experiments but the labelled cells exhibit fluorescence only in their somata and proximal dendrites. (4) Factors other than labelling intensity, e.g. double-labelling, preservation of the label, compatibility with other techniques and even economic reasons must be taken into consideration when a selection of visualization methods is to be made. |
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| المشرفين على المادة: | 0 (Fluorescent Dyes) 0 (Rhodamines) 0 (cholera toxin, B subunit-horseradish peroxidase) 4158-89-8 (tetramethylrhodamine isothiocyanate) 9012-63-9 (Cholera Toxin) EC 1.11.1.- (Horseradish Peroxidase) I223NX31W9 (Fluorescein-5-isothiocyanate) |
| تواريخ الأحداث: | Date Created: 19941101 Date Completed: 19950330 Latest Revision: 20210107 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/BF00162931 |
| PMID: | 7868356 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. | تدمد: | 0018-2214 |
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| DOI: | 10.1007/BF00162931 |