دورية أكاديمية
[Highly-sensitive nonradioactive detection of the tick-borne encephalitis virus].
| العنوان: | [Highly-sensitive nonradioactive detection of the tick-borne encephalitis virus]. |
|---|---|
| عنوان ترانسليتريتد: | Vysokochuvstvitel'naia neradioaktivnaia detektsiia virusa kleshchevogo éntsefalita. |
| المؤلفون: | Godovikova TS, Orlova TN, Dobrikova EIu, Shamanin VA, Zarytova VF, Vorob'eva NV, Serdiukova NA, Shamanina MIu, Petruseva IO, Pitsenko ND |
| المصدر: | Bioorganicheskaia khimiia [Bioorg Khim] 1994 Nov; Vol. 20 (11), pp. 1196-205. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Nauka Country of Publication: Russia (Federation) NLM ID: 7804941 Publication Model: Print Cited Medium: Print ISSN: 0132-3423 (Print) Linking ISSN: 01323423 NLM ISO Abbreviation: Bioorg Khim Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva, Nauka |
| مواضيع طبية MeSH: | Encephalitis Viruses, Tick-Borne/*isolation & purification, Base Sequence ; DNA Primers ; DNA Probes ; DNA, Complementary ; Encephalitis Viruses, Tick-Borne/genetics ; Genome, Viral ; Molecular Sequence Data ; Polymerase Chain Reaction ; RNA, Viral/analysis |
| مستخلص: | The non-radioactive reverse dot-blot method was used for the detection of tick-borne encephalitis virus (TBEV) in clinical specimens. The method involves reverse transcription (RT) and polymerase chain reaction (PCR) using a pair of biotin-labelled oligonucleotide primers. These primers flank a region in the gene of the envelope protein E, which is more conserved than other regions, and initiate the polymerisation with RNAs of all the investigated strains. The amplified cDNA was captured from solution on a solid support using complementary oligonucleotides covalently bound to a polyamide membrane. The biotin labels of the resulting hybrids were visualized by means of the streptavidin-horseradish peroxidase conjugate. The detection limit of the test was about 10(3)-10(4) molecules of target RNA. The sensitivity was comparable to that obtained by dot-hybridization of PCR-product with 32P-labelled DNA probe. The method was used for the detection of RNA in specimens of tick and blood. |
| المشرفين على المادة: | 0 (DNA Primers) 0 (DNA Probes) 0 (DNA, Complementary) 0 (RNA, Viral) |
| تواريخ الأحداث: | Date Created: 19941101 Date Completed: 19950403 Latest Revision: 20061115 |
| رمز التحديث: | 20231215 |
| PMID: | 7880179 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0132-3423 |
|---|