دورية أكاديمية
Quantitation of human immunodeficiency virus by culture and polymerase chain reaction in response to didanosine after long-term therapy with zidovudine.
| العنوان: | Quantitation of human immunodeficiency virus by culture and polymerase chain reaction in response to didanosine after long-term therapy with zidovudine. |
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| المؤلفون: | Katzenstein DA; Center for AIDS Research, Stanford University Medical Center, California., Winters M, Bubp J, Israelski D, Winger E, Merigan TC |
| المصدر: | The Journal of infectious diseases [J Infect Dis] 1994 Feb; Vol. 169 (2), pp. 416-9. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0413675 Publication Model: Print Cited Medium: Print ISSN: 0022-1899 (Print) Linking ISSN: 00221899 NLM ISO Abbreviation: J Infect Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2011- : Oxford : Oxford University Press Original Publication: 1904-2010 : Chicago, IL : University of Chicago Press |
| مواضيع طبية MeSH: | Didanosine/*administration & dosage , HIV Infections/*diagnosis , Zidovudine/*administration & dosage, Adult ; CD4-Positive T-Lymphocytes ; DNA, Viral/analysis ; HIV Core Protein p24/analysis ; HIV Infections/drug therapy ; HIV Infections/microbiology ; HIV Seropositivity/microbiology ; Humans ; Leukocyte Count ; Middle Aged ; Polymerase Chain Reaction ; Proviruses/chemistry ; RNA, Viral/analysis ; Time Factors |
| مستخلص: | Measurements of human immunodeficiency virus by quantitative RNA and DNA polymerase chain reaction (PCR), cell and plasma infectivity dilution cultures, and immune complex-disassociated p24 antigen-capture ELISA were made repeatedly in 10 subjects receiving long-term zidovudine treatment before and after therapy was changed to didanosine. Comparison of baseline assays showed that quantitative cell cultures, plasma RNA, and proviral DNA were measurable in all subjects and that cell culture results were significantly correlated with measures of nucleic acids. Plasma viremia (as indicated by culture) and p24 antigen were detected in three measurements in 3 of 8 and 6 of 10 subjects, respectively. Significant decreases in plasma RNA and cell dilution cultures from baseline were maintained for up to 6 months after initiation of didanosine therapy. These findings demonstrate a decrease in virus burden with the use of didanosine; however, continued detection of plasma RNA suggests that additional antiviral therapy will be required to suppress viral replication. |
| معلومات مُعتمدة: | AI-27762 United States AI NIAID NIH HHS |
| المشرفين على المادة: | 0 (DNA, Viral) 0 (HIV Core Protein p24) 0 (RNA, Viral) 4B9XT59T7S (Zidovudine) K3GDH6OH08 (Didanosine) |
| تواريخ الأحداث: | Date Created: 19940201 Date Completed: 19940323 Latest Revision: 20190512 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1093/infdis/169.2.416 |
| PMID: | 7906292 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0022-1899 |
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| DOI: | 10.1093/infdis/169.2.416 |