دورية أكاديمية
Beta 1-adrenergic and dopamine (D1)-receptors coupled to adenylyl cyclase activation in GT1 gonadotropin-releasing hormone neurosecretory cells.
| العنوان: | Beta 1-adrenergic and dopamine (D1)-receptors coupled to adenylyl cyclase activation in GT1 gonadotropin-releasing hormone neurosecretory cells. |
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| المؤلفون: | Findell PR; Institute of Immunology and Biological Sciences, Syntex Research, Palo Alto, California 94304., Wong KH, Jackman JK, Daniels DV |
| المصدر: | Endocrinology [Endocrinology] 1993 Feb; Vol. 132 (2), pp. 682-8. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0375040 Publication Model: Print Cited Medium: Print ISSN: 0013-7227 (Print) Linking ISSN: 00137227 NLM ISO Abbreviation: Endocrinology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2017- : New York : Oxford University Press Original Publication: Los Angeles, Calif. : Association for the Study of Internal Secretions, |
| مواضيع طبية MeSH: | Adenylyl Cyclases/*metabolism , Adrenergic beta-Agonists/*pharmacology , Adrenergic beta-Antagonists/*pharmacology , Dopamine Agents/*pharmacology , Gonadotropin-Releasing Hormone/*metabolism , Neurons/*metabolism , Receptors, Adrenergic, beta/*metabolism , Receptors, Dopamine D1/*metabolism, Animals ; Benzazepines/analogs & derivatives ; Benzazepines/metabolism ; Benzazepines/pharmacology ; Binding, Competitive ; Cell Line ; Dopamine/pharmacology ; Hypothalamus ; Iodine Radioisotopes ; Iodocyanopindolol ; Isoproterenol/pharmacology ; Kinetics ; Mice ; Pindolol/analogs & derivatives ; Pindolol/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Receptors, Adrenergic, beta/drug effects ; Receptors, Adrenergic, beta/genetics ; Receptors, Dopamine D1/drug effects ; Receptors, Dopamine D1/genetics ; Sulpiride/pharmacology |
| مستخلص: | Binding sites labeled by the beta-adrenergic receptor radioligand (-)-[125I]iodocyanopindolol ([125I]ICYP) and the selective D1-subtype dopamine (DA) receptor radioligand (+)-[125I]SCH 23982 were identified on immortalized hypothalamic GnRH neurons (GT1-7 cell lines). Saturation analyses in crude particulate suspensions of GT1 cells described high affinity and low capacity binding sites for [125I]ICYP (Kd, 41 pM; binding capacity, 25 fmol/mg protein) and [125I]SCH 23982 (Kd, 320 pM; binding capacity, 23 fmol/mg protein). These binding sites were further characterized in competition assays using a variety of agonists and antagonists selective for either beta-adrenergic or DA receptor subtypes. The pharmacological profiles of [125I]ICYP and [125I]SCH 23982 binding obtained from these studies indicated that the radioligands were labeling beta 1-adrenergic and D1-dopaminergic receptor sites, respectively. Northern blot analyses of purified GT1 cell mRNA documented the expression of D1-dopaminergic and beta 1-adrenergic receptor mRNAs. beta 2-Adrenergic receptor mRNA was not identified. All three transcripts were detected in mouse brain mRNA. Both beta 1-adrenergic and D1-receptors were discovered to be positively coupled to adenylyl cyclase. DA and the beta-adrenergic agonist isoproterenol each provoked a rapid and marked stimulation of adenylyl cyclase activity in GT1 cell membrane suspensions. Subtype-selective beta-adrenergic and DA receptor antagonists were used to inhibit isoproterenol- and DA-stimulated adenylyl cyclase activities. Their relative potencies indicated that the isoproterenol stimulation was mediated via the beta 1-adrenergic receptor. The DA-stimulated adenylyl cyclase activity was mediated via the D1-DA receptor. These studies have identified functional beta 1-adrenergic and D1-dopaminergic receptors positively coupled to adenylyl cyclase on GT1 GnRH neurosecretory cells. The existence of these receptors suggests that the noradrenergic and dopaminergic regulation of gonadotropin secretion may be mediated at least in part via direct synapses on GnRH neurons. |
| المشرفين على المادة: | 0 (8-iodo-2,3,4,5-tetrahydro-3-methyl-5-phenyl-1H-3-benzazepine-7-ol) 0 (Adrenergic beta-Agonists) 0 (Adrenergic beta-Antagonists) 0 (Benzazepines) 0 (Dopamine Agents) 0 (Iodine Radioisotopes) 0 (RNA, Messenger) 0 (Receptors, Adrenergic, beta) 0 (Receptors, Dopamine D1) 33515-09-2 (Gonadotropin-Releasing Hormone) 7MNE9M8287 (Sulpiride) 83498-72-0 (Iodocyanopindolol) BJ4HF6IU1D (Pindolol) EC 4.6.1.1 (Adenylyl Cyclases) L628TT009W (Isoproterenol) VTD58H1Z2X (Dopamine) |
| تواريخ الأحداث: | Date Created: 19930201 Date Completed: 19930302 Latest Revision: 20181130 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1210/endo.132.2.8093877 |
| PMID: | 8093877 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0013-7227 |
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| DOI: | 10.1210/endo.132.2.8093877 |