دورية أكاديمية
Establishment of cell lines from B-cell precursor acute lymphoblastic leukemia.
| العنوان: | Establishment of cell lines from B-cell precursor acute lymphoblastic leukemia. |
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| المؤلفون: | Zhang LQ; University of Chicago Department of Pediatrics, IL., Downie PA, Goodell WR, McCabe NR, LeBeau MM, Morgan R, Sklar J, Raimondi SC, Miley D, Goldberg A, et. al. |
| المصدر: | Leukemia [Leukemia] 1993 Nov; Vol. 7 (11), pp. 1865-74. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group, Specialist Journals Country of Publication: England NLM ID: 8704895 Publication Model: Print Cited Medium: Print ISSN: 0887-6924 (Print) Linking ISSN: 08876924 NLM ISO Abbreviation: Leukemia Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2000- : London : Nature Publishing Group, Specialist Journals Original Publication: [Baltimore, Md.] : Williams & Wilkins, [c1987- |
| مواضيع طبية MeSH: | Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/*pathology , Precursor Cell Lymphoblastic Leukemia-Lymphoma/*pathology, Adolescent ; Adult ; Cell Division/drug effects ; Child ; Child, Preschool ; Female ; Gene Rearrangement, B-Lymphocyte ; Humans ; Immunophenotyping ; Karyotyping ; Leucine/analogs & derivatives ; Leucine/pharmacology ; Leukocytes, Mononuclear/drug effects ; Leukocytes, Mononuclear/pathology ; Male ; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/genetics ; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/immunology ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/immunology ; Tumor Cells, Cultured/drug effects ; Tumor Cells, Cultured/immunology ; Tumor Cells, Cultured/pathology |
| مستخلص: | In an attempt to establish permanent cell lines from children with B-cell precursor acute lymphoblastic leukemia (BCP-ALL), 123 clinical samples from 117 patients were cultured in vitro. Using a method which was successful for the growth of ALL with T-cell phenotype, 3% (2/74) of BCP-ALL samples from patients at diagnosis and 31% (9/29) of BCP-ALL samples from patients at relapse were established as cell lines. However, in most cultures, leukemic cells survived for only a few weeks and the majority of viable cells present after 28 days of culture were esterase-positive mononuclear cells. Based on the hypothesis that mononuclear cells inhibited leukemic cell growth, we evaluated the effect of a monocyte toxin, L-leucine methyl ester (Leu-OMe), on the growth of four frozen BCP-ALL samples. Thawed leukemic cells treated with Leu-OMe, but not untreated control cells, proliferated in three samples and one new cell line was established. Subsequently, when Leu-OMe was added to fresh leukemia cells in culture, leukemic cell lines were grown from 29% (4/14) of samples at diagnosis and 66% (4/6) of relapse samples. Overall, 20 BCP-ALL cell lines were established, all were Epstein-Barr virus (EBV)-negative, and authenticity of each cell line was verified by a direct comparison of the immunophenotype, karyotype, and genotype with the patient's tumor cells. This improved method of cell culture permits a higher success rate of cell line establishment from patients with BCP-ALL thereby aiding in analysis of B-lymphocyte transformation and neoplasia. |
| المشرفين على المادة: | 2666-93-5 (leucine methyl ester) GMW67QNF9C (Leucine) |
| تواريخ الأحداث: | Date Created: 19931101 Date Completed: 19931210 Latest Revision: 20131121 |
| رمز التحديث: | 20240627 |
| PMID: | 8231254 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0887-6924 |
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