دورية أكاديمية
FABMS/derivatisation strategies for the analysis of heparin-derived oligosaccharides.
| العنوان: | FABMS/derivatisation strategies for the analysis of heparin-derived oligosaccharides. |
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| المؤلفون: | Khoo KH; Department of Biochemistry, Imperial College, London, United Kingdom., Morris HR, McDowell RA, Dell A, Maccarana M, Lindahl U |
| المصدر: | Carbohydrate research [Carbohydr Res] 1993 Jun 21; Vol. 244 (2), pp. 205-23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0043535 Publication Model: Print Cited Medium: Print ISSN: 0008-6215 (Print) Linking ISSN: 00086215 NLM ISO Abbreviation: Carbohydr Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Amsterdam. |
| مواضيع طبية MeSH: | Heparin/*chemistry , Oligosaccharides/*analysis, Acetylation ; Carbohydrate Sequence ; Deamination ; Methanol/chemistry ; Methylation ; Molecular Sequence Data ; Molecular Structure ; Spectrometry, Mass, Fast Atom Bombardment |
| مستخلص: | Derivatisation/FABMS strategies applicable to the structure analysis of low microgram quantities of heparin-derived oligosaccharides are described. Negative and positive FAB data from permethyl derivatives and positive FAB data from the products of subsequent methanolysis are reported for sulfated tetrasaccharides prepared by nitrous acid degradation of heparin. The preparation and FAB behaviour of acetylated derivatives of sulfated oligosaccharides are described for the first time, and the stability of the sulfate groups to base-catalysed acetylation is demonstrated. The acetylation/FABMS methodology, which yields high quality data, shows promise for the characterisation of a wide range of sulfated glycoconjugates. |
| معلومات مُعتمدة: | United Kingdom Wellcome Trust |
| المشرفين على المادة: | 0 (Oligosaccharides) 9005-49-6 (Heparin) Y4S76JWI15 (Methanol) |
| تواريخ الأحداث: | Date Created: 19930621 Date Completed: 19930916 Latest Revision: 20190813 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1016/0008-6215(83)85002-2 |
| PMID: | 8348550 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0008-6215 |
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| DOI: | 10.1016/0008-6215(83)85002-2 |