دورية أكاديمية
Oxidative stress in fish cells: in vitro studies.
| العنوان: | Oxidative stress in fish cells: in vitro studies. |
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| المؤلفون: | Babich H; Stern College for Women, Yeshiva University, Department of Biological Sciences, New York, New York 10016., Palace MR, Stern A |
| المصدر: | Archives of environmental contamination and toxicology [Arch Environ Contam Toxicol] 1993 Feb; Vol. 24 (2), pp. 173-8. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: United States NLM ID: 0357245 Publication Model: Print Cited Medium: Print ISSN: 0090-4341 (Print) Linking ISSN: 00904341 NLM ISO Abbreviation: Arch Environ Contam Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, Springer-Verlag. |
| مواضيع طبية MeSH: | Glutathione/*metabolism , Hydrogen Peroxide/*toxicity , Paraquat/*toxicity, 3T3 Cells/drug effects ; Animals ; Cell Line ; Fishes ; Humans ; Lethal Dose 50 ; Mice ; Oxidation-Reduction ; Tumor Cells, Cultured |
| مستخلص: | Bluegill sunfish BF-2 fibroblasts were used in the neutral red (NR) cytotoxicity assay to discern the toxicities of hydrogen peroxide (H2O2) and paraquat as indicated by their abilities to induce oxidative stress. The toxicity of H2O2 was markedly enhanced in BF-2 cells treated with the glutathione depleting agents, buthionine sulfoximine (BSO), maleic acid, and chlorodinitrobenzene; similar treatments did not sensitize the BF-2 cells to paraquat, a redox cycling xenobiotic. BSO treated BF-2 cells, however, were sensitized to nitrofurantoin, also a redox cycling chemical. Diethyldithiocarbamate, an inhibitor of superoxide dismutase, only weakly enhanced the sensitivity of the BF-2 cells to H2O2 and paraquat. 1,10-Phenanthroline, a chelator of Fe2+, reduced the cytotoxicity of H2O2 and paraquat, presumably by preventing hydroxyl radical formation in the Fenton reaction. Quin 2 AM, an intracellular chelator of Ca2+, markedly lessened the toxicity of H2O2, but not of paraquat; EGTA, an extracellular chelator of Ca2+, had no effect on the toxicity of H2O2 or paraquat. Apparently, perturbation of intracellular Ca2+ homeostasis is involved in H2O2 toxicity. For comparative purposes, some studies were performed with fathead minnow FHM epithelioid cells, BALB/c mouse 3T3 fibroblasts, and human HepG2 hepatoma cells. The BF-2 fibroblast/NR cytotoxicity red assay was shown to be a suitable model to study oxidative stress in fish. |
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| معلومات مُعتمدة: | ESO3425 United States ES NIEHS NIH HHS |
| المشرفين على المادة: | BBX060AN9V (Hydrogen Peroxide) GAN16C9B8O (Glutathione) PLG39H7695 (Paraquat) |
| تواريخ الأحداث: | Date Created: 19930201 Date Completed: 19930506 Latest Revision: 20190904 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/BF01141344 |
| PMID: | 8466299 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 0090-4341 |
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| DOI: | 10.1007/BF01141344 |