دورية أكاديمية
Monoclonal immunoglobulin A derived from peritoneal B cells is encoded by both germ line and somatically mutated VH genes and is reactive with commensal bacteria.
| العنوان: | Monoclonal immunoglobulin A derived from peritoneal B cells is encoded by both germ line and somatically mutated VH genes and is reactive with commensal bacteria. |
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| المؤلفون: | Bos NA; Department of Histology and Cell Biology, University of Groningen, The Netherlands., Bun JC, Popma SH, Cebra ER, Deenen GJ, van der Cammen MJ, Kroese FG, Cebra JJ |
| المصدر: | Infection and immunity [Infect Immun] 1996 Feb; Vol. 64 (2), pp. 616-23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0246127 Publication Model: Print Cited Medium: Print ISSN: 0019-9567 (Print) Linking ISSN: 00199567 NLM ISO Abbreviation: Infect Immun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington, DC : American Society For Microbiology Original Publication: [Bethesda, Md.] American Society for Microbiology. |
| مواضيع طبية MeSH: | Genes, Immunoglobulin*, Antibodies, Monoclonal/*genetics , B-Lymphocytes/*immunology , Bacteria/*immunology , Feces/*microbiology , Immunoglobulin A/*genetics , Immunoglobulin Heavy Chains/*genetics , Immunoglobulin Variable Region/*genetics, Animals ; Base Sequence ; Flow Cytometry ; Hybridomas/immunology ; Immunoglobulin A/immunology ; Mice ; Mice, Inbred BALB C ; Mice, SCID ; Molecular Sequence Data ; Mutation ; Peritoneal Cavity/cytology |
| مستخلص: | We transferred peritoneal cells from BALB/c mice into C.B17 scid/scid mice. Six to eight months after injection, only cells with the B1 phenotype were retained in the spleens and peritoneal cavities of these mice. The lamina propria of the intestine contained many peritoneal, donor-derived, immunoglobulin A (IgA)-producing cells. The mesenteric lymph nodes of these mice were found to be a major site of proliferation and generation of IgA plasmablasts. We established eight IgA-producing hybridomas from the mesenteric lymph nodes of such mice, and all the hybridomas reacted with different but partially overlapping fecal bacterial populations. Cloning and sequencing of the VH genes of these hybridomas showed that two hybridomas utilized germ line-encoded VH genes while the VH genes of the six hybridomas showed somatic mutations, some of which are indicative of an antigen-driven selection process. |
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| سلسلة جزيئية: | GENBANK X94415; X94416; X94417; X94418; X94419; X94420; X94421; X94422 |
| المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (Immunoglobulin A) 0 (Immunoglobulin Heavy Chains) 0 (Immunoglobulin Variable Region) |
| تواريخ الأحداث: | Date Created: 19960201 Date Completed: 19960222 Latest Revision: 20210526 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC173810 |
| DOI: | 10.1128/iai.64.2.616-623.1996 |
| PMID: | 8550216 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0019-9567 |
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| DOI: | 10.1128/iai.64.2.616-623.1996 |