دورية أكاديمية
bcl-xs gene therapy induces apoptosis of human mammary tumors in nude mice.
| العنوان: | bcl-xs gene therapy induces apoptosis of human mammary tumors in nude mice. |
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| المؤلفون: | Ealovega MW; Department of Internal Medicine, University of Michigan Comprehensive Cancer Center, Ann Arbor 48109-0724, USA., McGinnis PK, Sumantran VN, Clarke MF, Wicha MS |
| المصدر: | Cancer research [Cancer Res] 1996 May 01; Vol. 56 (9), pp. 1965-9. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association for Cancer Research Country of Publication: United States NLM ID: 2984705R Publication Model: Print Cited Medium: Print ISSN: 0008-5472 (Print) Linking ISSN: 00085472 NLM ISO Abbreviation: Cancer Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Baltimore, Md. : American Association for Cancer Research Original Publication: Chicago [etc.] |
| مواضيع طبية MeSH: | Genetic Therapy* , Proto-Oncogene Proteins c-bcl-2*, Apoptosis/*genetics , Mammary Neoplasms, Experimental/*therapy , Proto-Oncogene Proteins/*genetics, Animals ; Female ; Gene Transfer Techniques ; Humans ; Mammary Neoplasms, Experimental/genetics ; Mammary Neoplasms, Experimental/pathology ; Mice ; Mice, Nude ; Neoplasm Transplantation ; bcl-X Protein |
| مستخلص: | Bcl-xs is a dominant negative repressor of Bcl-2 and Bcl-xL, both of which inhibit apoptosis. We used a replication-deficient adenoviral vector to transiently overexpress Bcl-xs in MCF-7 human breast cancer cells, which overexpress Bcl-xL. Infection with this vector induced apoptosis in vitro. We then determined the effects of intratumoral injection of bcl-xs adenovirus on solid MCF-7 tumors in nude mice. Tumors injected four times with the bcl-xs adenovirus showed a 50% reduction in size. Using terminal transferase-mediated dUTP-digoxigenin nick end labeling, we observed apoptotic cells at sites of bcl-xs adenoviral injection. These experiments demonstrate the feasibility of using bcl-xs gene therapy to induce apoptosis in human breast tumors. |
| معلومات مُعتمدة: | CA-61777 United States CA NCI NIH HHS; CA-64556 United States CA NCI NIH HHS |
| المشرفين على المادة: | 0 (BCL2L1 protein, human) 0 (Bcl2l1 protein, mouse) 0 (Proto-Oncogene Proteins) 0 (Proto-Oncogene Proteins c-bcl-2) 0 (bcl-X Protein) |
| تواريخ الأحداث: | Date Created: 19960501 Date Completed: 19960612 Latest Revision: 20121115 |
| رمز التحديث: | 20240627 |
| PMID: | 8616832 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0008-5472 |
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