دورية أكاديمية
Determination of phenylbutazone and oxyphenbutazone in plasma and urine samples of horses by high-performance liquid chromatography and gas chromatography-mass spectrometry.
| العنوان: | Determination of phenylbutazone and oxyphenbutazone in plasma and urine samples of horses by high-performance liquid chromatography and gas chromatography-mass spectrometry. |
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| المؤلفون: | Neto LM; Antidoping Laboratory, Jockey Club de São Paulo, Brazil., Andraus MH, Salvadori MC |
| المصدر: | Journal of chromatography. B, Biomedical applications [J Chromatogr B Biomed Appl] 1996 Apr 12; Vol. 678 (2), pp. 211-8. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9421796 Publication Model: Print Cited Medium: Print ISSN: 1572-6495 (Print) Linking ISSN: 15726495 NLM ISO Abbreviation: J Chromatogr B Biomed Appl Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Netherlands : Elsevier, c1994-c1996. |
| مواضيع طبية MeSH: | Chromatography, High Pressure Liquid/*methods , Gas Chromatography-Mass Spectrometry/*methods , Horses/*metabolism , Oxyphenbutazone/*analysis , Phenylbutazone/*analysis, Acetic Acid ; Animals ; Chromatography, High Pressure Liquid/statistics & numerical data ; Chromatography, Thin Layer ; Female ; Gas Chromatography-Mass Spectrometry/statistics & numerical data ; Methanol ; Oxyphenbutazone/blood ; Oxyphenbutazone/urine ; Phenylbutazone/blood ; Phenylbutazone/urine |
| مستخلص: | A method is described for the qualitative and quantitative determination of phenylbutazone and oxyphenbutazone in horse urine and plasma samples viewing antidoping control. A horse was administered intravenously with 3 g of phenylbutazone. For the qualitative determination, a screening by HPLC was performed after acidic extraction of the urine samples and the confirmation process was realized by GC-MS. Using the proposed method it was possible to detect phenylbutazone and oxyphenbutazone in urine for up to 48 and 120 h, respectively. For the quantitation of these drugs the plasma was deproteinized with acetonitrile and 20 microliters were injected directly into the HPLC system equipped with a UV detector and LiChrospher RP-18 column. The mobile phase used was 0.01 M acetic acid in methanol (45:55, v/v). The limit of detection was 0.5 microgram/ml for phenylbutazone and oxyphenbutazone and the limit of quantitation was 1.0 microgram/ml for both drugs. Using the proposed method it was possible to quantify phenylbutazone up to 30 h and oxyphenbutazone up to 39 h after administration. |
| المشرفين على المادة: | GN5P7K3T8S (Phenylbutazone) H806S4B3NS (Oxyphenbutazone) Q40Q9N063P (Acetic Acid) Y4S76JWI15 (Methanol) |
| تواريخ الأحداث: | Date Created: 19960412 Date Completed: 19970128 Latest Revision: 20190914 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/0378-4347(95)00508-0 |
| PMID: | 8738024 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1572-6495 |
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| DOI: | 10.1016/0378-4347(95)00508-0 |