دورية أكاديمية
Retrovirus-mediated gene transfer into salivary glands in vivo.
| العنوان: | Retrovirus-mediated gene transfer into salivary glands in vivo. |
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| المؤلفون: | Barka T; Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, City University of New York, NY 10029, USA., Van der Noen HM |
| المصدر: | Human gene therapy [Hum Gene Ther] 1996 Mar 20; Vol. 7 (5), pp. 613-8. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: M.A. Liebert Country of Publication: United States NLM ID: 9008950 Publication Model: Print Cited Medium: Print ISSN: 1043-0342 (Print) Linking ISSN: 10430342 NLM ISO Abbreviation: Hum Gene Ther Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York : M.A. Liebert, c1990- |
| مواضيع طبية MeSH: | Gene Transfer Techniques* , Genetic Vectors*, Retroviridae/*genetics , Salivary Glands/*metabolism, Adrenergic beta-Agonists/pharmacology ; Animals ; Cell Division/drug effects ; Cell Line ; Female ; Gene Expression ; Genetic Therapy/methods ; Histocytochemistry ; Isoproterenol/pharmacology ; Rats ; Rats, Sprague-Dawley ; Retroviridae/metabolism ; Salivary Glands/virology ; Submandibular Gland/metabolism ; Transduction, Genetic ; beta-Galactosidase/genetics ; beta-Galactosidase/metabolism |
| مستخلص: | In the present report, we show prolonged expression of beta-galactosidase (beta-Gal) in the acinar cells of the submandibular and sublingual glands of rats following retrograde ductal injection of the retroviral vector BAG. To facilitate integration of viral DNA, cell division in the gland was induced by the administration of the beta-adrenergic agonist isoproterenol prior to the delivery of the vector. The frequency of cells stained for beta-Gal was higher if the virus was injected 4-20 hr after the two injections of isoproterenol given 24 hr apart than after the injection of only one dose of the drug. Without stimulation of cell division, no integration of the viral DNA was observed. Expression of the marker enzyme was observed up to 43 days, the limit of the observation period. The data indicate that salivary glands are potential targets of retrovirus-mediated gene transfer for somatic gene therapy. |
| معلومات مُعتمدة: | HL39419 United States HL NHLBI NIH HHS |
| المشرفين على المادة: | 0 (Adrenergic beta-Agonists) EC 3.2.1.23 (beta-Galactosidase) L628TT009W (Isoproterenol) |
| تواريخ الأحداث: | Date Created: 19960320 Date Completed: 19961024 Latest Revision: 20131121 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1089/hum.1996.7.5-613 |
| PMID: | 8845386 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1043-0342 |
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| DOI: | 10.1089/hum.1996.7.5-613 |