دورية أكاديمية
أعرض في EDS

Modulation of L-type Ca2+ current by fast and slow Ca2+ buffering in guinea pig ventricular cardiomyocytes.

التفاصيل البيبلوغرافية
العنوان: Modulation of L-type Ca2+ current by fast and slow Ca2+ buffering in guinea pig ventricular cardiomyocytes.
المؤلفون: You Y; Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia, Canada., Pelzer DJ, Pelzer S
المصدر: Biophysical journal [Biophys J] 1997 Jan; Vol. 72 (1), pp. 175-87.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 0370626 Publication Model: Print Cited Medium: Print ISSN: 0006-3495 (Print) Linking ISSN: 00063495 NLM ISO Abbreviation: Biophys J Subsets: MEDLINE
أسماء مطبوعة: Publication: Cambridge, MA : Cell Press
Original Publication: New York, Published by Rockefeller University Press [etc.] for the Biophysical Society.
مواضيع طبية MeSH: Calcium/*physiology , Calcium Channels/*physiology , Egtazic Acid/*analogs & derivatives , Heart/*physiology, Animals ; Calcium Channels/chemistry ; Calcium Channels/drug effects ; Calcium Channels, L-Type ; Cell Membrane/drug effects ; Cell Membrane/physiology ; Cell Membrane/ultrastructure ; Cells, Cultured ; Chelating Agents/pharmacology ; Dose-Response Relationship, Drug ; Egtazic Acid/pharmacology ; Guinea Pigs ; Heart Ventricles ; Kinetics ; Membrane Potentials/drug effects ; Models, Structural ; Time Factors
مستخلص: Free Ca2+ near Ca2+ channel pores is expected to be lower in cardiomyocytes dialyzed with bis-(o-amino-phenoxy)-ethane-N,N,N',N'-tetraacetic acid (BAPTA) than with ethyleneglycol-bis-(beta-aminoethyl)-N,N,N',N'-tetraacetic acid (EGTA) because BAPTA chelates incoming Ca2+ more rapidly. The consequences of intracellular Ca2+ buffering by BAPTA (0.2-60 mM) and by EGTA (0.2-67 mM) on whole-cell L-type Ca2+ current (ICa,L) were investigated in voltage-clamped guinea pig ventricular cardiomyocytes; bulk cytoplasmic free Ca2+ (Cac2+) was monitored using the fluorescent Ca2+ indicator indo-1. ICa,L was augmented by approximately 12-fold when BAPTA in the cell dialysate was increased from 0.2 to 50 mM (half-maximal stimulation at 31 mM), whereas elevating internal EGTA from 0.2 to 67 mM increased ICa,L only by approximately 2-fold. Cac2+ was < 20 nM with internal BAPTA or EGTA > or = 20 mM. While EGTA up to 67 mM had only an insignificant inhibitory effect on the stimulation of ICa,L by 3 microM forskolin, ICa,L in 50 mM BAPTA-dialyzed myocytes was insensitive to forskolin-induced elevation of adenosine 3',5'-cyclic monophosphate (cAMP); conversely, ICa,L in cAMP-loaded cells was unresponsive to BAPTA dialysis. Cell dialysis with BAPTA, but not with EGTA, accelerated the slow component of ICa,L inactivation (tau S) without affecting its fast component (tau F), resembling the effects of cAMP-dependent phosphorylation. BAPTA-stimulated ICa,L was inhibited by acetylcholine and by the cAMP-dependent protein kinase (PKA) blocker H-89. These results suggest that BAPTA-induced lowering of peri-channel Ca2+ stimulates cAMP synthesis and channel phosphorylation by disinhibiting Ca(2+)-sensitive adenylyl cyclase.
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المشرفين على المادة: 0 (Calcium Channels)
0 (Calcium Channels, L-Type)
0 (Chelating Agents)
526U7A2651 (Egtazic Acid)
K22DDW77C0 (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid)
SY7Q814VUP (Calcium)
تواريخ الأحداث: Date Created: 19970101 Date Completed: 19970527 Latest Revision: 20181113
رمز التحديث: 20240627
مُعرف محوري في PubMed: PMC1184306
DOI: 10.1016/S0006-3495(97)78656-9
PMID: 8994602
قاعدة البيانات: MEDLINE
الوصف
تدمد:0006-3495
DOI:10.1016/S0006-3495(97)78656-9